A highly conserved insulin-like growth factor-binding protein (IGFBP-5) is expressed during myoblast differentiation

Payton L. James, Stephanie B. Jones, Walker H. Busby, David R. Clemmons, Peter Rotwein

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Insulin-like growth factor-binding proteins (IGFBPs) are a family of secreted proteins that bind insulin-like growth factors I and II (IGFs I and II) and are capable of modulating IGF actions on target cells. We have shown previously that C2 myoblasts secrete a single ∼29-kDa IGFBP during their terminal differentiation (Tollefsen, S. E., Lajara, R., McCusker, R. H., Clemmons, D. R., and Rotwein, P. (1989) J. Biol. Chem. 264, 13810-13817). In this study, we have purified the protein from C2 cell-conditioned media by conventional and IGF-affinity chromatography, cloned its cDNA by PCR-based and traditional library screening, and identified it as mouse IGFBP-5. The resultant 5561 nucleotide cDNA encodes a 252-amino acid mature protein (predicted Mr ∼28,400) that is 97% identical to rat and human IGFBP-5. In differentiating C2 myoblasts and in F3 azamyoblasts the >6-kilobase IGFBP-5 mRNA accumulates concomitantly with induction of myogenin mRNA, an early marker of muscle differentiation. Ligand blot analysis shows that IGFBP-5 protein is secreted within 12 h of the onset of differentiation in these cells and that it is the only IGFBP produced in several fusing skeletal muscle cell lines. In vivo, IGFBP-5 transcripts are expressed in a variety of mouse tissues including striated muscle, but, unlike other IGFBPs, it is barely detectable in liver. IGFBP-5 is more conserved than other IGFBPs in mammals; its conserved structure and sequence also extends to non-mammalian vertebrates. Hybridization of a mouse BP5 coding region probe to RNA from several chicken and Xenopus tissues demonstrated similarly sized transcripts in these species. A partial Xenopus cDNA is identical in 38/45 deduced amino acids to the mammalian proteins. Identification of an IGF-binding protein that is produced during myoblast differentiation provides a model system in which to study the potential modulatory role of IGFBPs in development.

Original languageEnglish (US)
Pages (from-to)22305-22312
Number of pages8
JournalJournal of Biological Chemistry
Volume268
Issue number30
StatePublished - Oct 25 1993
Externally publishedYes

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Insulin-Like Growth Factor Binding Protein 5
Insulin-Like Growth Factor Binding Proteins
Myoblasts
Muscle
Insulin-Like Growth Factor II
Complementary DNA
Xenopus
Proteins
Insulin-Like Growth Factor I
Tissue
Myogenin
RNA Probes
Affinity chromatography
Amino Acids
Messenger RNA
Mammals
Striated Muscle
Conserved Sequence
Differentiation Antigens
Conditioned Culture Medium

ASJC Scopus subject areas

  • Biochemistry

Cite this

James, P. L., Jones, S. B., Busby, W. H., Clemmons, D. R., & Rotwein, P. (1993). A highly conserved insulin-like growth factor-binding protein (IGFBP-5) is expressed during myoblast differentiation. Journal of Biological Chemistry, 268(30), 22305-22312.

A highly conserved insulin-like growth factor-binding protein (IGFBP-5) is expressed during myoblast differentiation. / James, Payton L.; Jones, Stephanie B.; Busby, Walker H.; Clemmons, David R.; Rotwein, Peter.

In: Journal of Biological Chemistry, Vol. 268, No. 30, 25.10.1993, p. 22305-22312.

Research output: Contribution to journalArticle

James, PL, Jones, SB, Busby, WH, Clemmons, DR & Rotwein, P 1993, 'A highly conserved insulin-like growth factor-binding protein (IGFBP-5) is expressed during myoblast differentiation', Journal of Biological Chemistry, vol. 268, no. 30, pp. 22305-22312.
James, Payton L. ; Jones, Stephanie B. ; Busby, Walker H. ; Clemmons, David R. ; Rotwein, Peter. / A highly conserved insulin-like growth factor-binding protein (IGFBP-5) is expressed during myoblast differentiation. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 30. pp. 22305-22312.
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