Abstract
The spinocerebellar ataxia type 2 (SCA2) gene has been localized to chromosome 12q24.1. To characterize this region and to aid in the identification of the SCA2 gene, we have constructed a 3.9-Mb physical map, which covers markers D12S1328 and D12S1329 known to flank the gene. The map comprises a contig of 84 overlapping yeast artificial chromosomes (YACs), P1 artificial chromosomes (PACs), and bacterial artificial chromosomes (BACs) onto which we placed 82 PCR markers. We localized eight genes and expressed sequence tags on this map, many of which had not been precisely mapped before. In contrast to YACs, which showed a high degree of chimerism and deletions in this region, PACs and BACs were stable. Only 1 in 65 PACs contained a small deletion, and 2 in 18 BACs were chimeric. The high- resolution physical map, which was used in the identification of the SCA2 gene, will be useful for the positional cloning of other disease genes mapped to this region.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 321-329 |
| Number of pages | 9 |
| Journal | Genomics |
| Volume | 44 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 15 1997 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
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A high-resolution PAC and BAC map of the SCA2 region. / Nechiporuk, Tamilla; Nechiporuk, Alex; Sahba, Soodabeh; Figueroa, Karla; Shibata, Hiroki; Chen, Xiao Ning; Korenberg, Julie R.; De Jong, Pieter; Pulst, Stefan M.
In: Genomics, Vol. 44, No. 3, 15.09.1997, p. 321-329.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A high-resolution PAC and BAC map of the SCA2 region
AU - Nechiporuk, Tamilla
AU - Nechiporuk, Alex
AU - Sahba, Soodabeh
AU - Figueroa, Karla
AU - Shibata, Hiroki
AU - Chen, Xiao Ning
AU - Korenberg, Julie R.
AU - De Jong, Pieter
AU - Pulst, Stefan M.
PY - 1997/9/15
Y1 - 1997/9/15
N2 - The spinocerebellar ataxia type 2 (SCA2) gene has been localized to chromosome 12q24.1. To characterize this region and to aid in the identification of the SCA2 gene, we have constructed a 3.9-Mb physical map, which covers markers D12S1328 and D12S1329 known to flank the gene. The map comprises a contig of 84 overlapping yeast artificial chromosomes (YACs), P1 artificial chromosomes (PACs), and bacterial artificial chromosomes (BACs) onto which we placed 82 PCR markers. We localized eight genes and expressed sequence tags on this map, many of which had not been precisely mapped before. In contrast to YACs, which showed a high degree of chimerism and deletions in this region, PACs and BACs were stable. Only 1 in 65 PACs contained a small deletion, and 2 in 18 BACs were chimeric. The high- resolution physical map, which was used in the identification of the SCA2 gene, will be useful for the positional cloning of other disease genes mapped to this region.
AB - The spinocerebellar ataxia type 2 (SCA2) gene has been localized to chromosome 12q24.1. To characterize this region and to aid in the identification of the SCA2 gene, we have constructed a 3.9-Mb physical map, which covers markers D12S1328 and D12S1329 known to flank the gene. The map comprises a contig of 84 overlapping yeast artificial chromosomes (YACs), P1 artificial chromosomes (PACs), and bacterial artificial chromosomes (BACs) onto which we placed 82 PCR markers. We localized eight genes and expressed sequence tags on this map, many of which had not been precisely mapped before. In contrast to YACs, which showed a high degree of chimerism and deletions in this region, PACs and BACs were stable. Only 1 in 65 PACs contained a small deletion, and 2 in 18 BACs were chimeric. The high- resolution physical map, which was used in the identification of the SCA2 gene, will be useful for the positional cloning of other disease genes mapped to this region.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031572301&partnerID=8YFLogxK
U2 - 10.1006/geno.1997.4886
DO - 10.1006/geno.1997.4886
M3 - Article
C2 - 9325053
AN - SCOPUS:0031572301
VL - 44
SP - 321
EP - 329
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 3
ER -