A herpes simplex virus gD-YFP fusion glycoprotein is transported separately from viral capsids in neuronal axons

Aleksandra Snyder, Birgitte Bruun, Helena M. Browne, David Johnson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Two models describing how alphaherpesviruses exit neurons differ with respect to whether nucleocapsids and envelope glycoproteins travel toward axon termini separately or as assembled enveloped virions. Recently, a pseudorabies virus glycoprotein D (gD)-green fluorescent protein fusion was found to colocalize with viral capsids, supporting anterograde transport of enveloped virions. Previous antibody staining experiments demonstrated that herpes simplex virus (HSV) glycoproteins and capsids are separately transported in axons. Here, we generated an HSV expressing a gD-yellow fluorescent protein (YFP) fusion and found that gD-YFP and capsids were transported separately in neuronal axons. Anti-gD antibodies colocalized with gD-YFP, indicating that gD-YFP behaves like wild-type HSV gD.

Original languageEnglish (US)
Pages (from-to)8337-8340
Number of pages4
JournalJournal of Virology
Volume81
Issue number15
DOIs
StatePublished - Aug 2007

Fingerprint

herpes simplex
capsid
Capsid
Simplexvirus
axons
Axons
glycoproteins
Glycoproteins
viruses
Proteins
Virion
virion
Nucleocapsid
yellow fluorescent protein
Antibodies
nucleocapsid
Capsid Proteins
antibodies
Suid herpesvirus 1
Green Fluorescent Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

A herpes simplex virus gD-YFP fusion glycoprotein is transported separately from viral capsids in neuronal axons. / Snyder, Aleksandra; Bruun, Birgitte; Browne, Helena M.; Johnson, David.

In: Journal of Virology, Vol. 81, No. 15, 08.2007, p. 8337-8340.

Research output: Contribution to journalArticle

Snyder, Aleksandra ; Bruun, Birgitte ; Browne, Helena M. ; Johnson, David. / A herpes simplex virus gD-YFP fusion glycoprotein is transported separately from viral capsids in neuronal axons. In: Journal of Virology. 2007 ; Vol. 81, No. 15. pp. 8337-8340.
@article{17c1dee4d9704ef095b75bf91e3efacb,
title = "A herpes simplex virus gD-YFP fusion glycoprotein is transported separately from viral capsids in neuronal axons",
abstract = "Two models describing how alphaherpesviruses exit neurons differ with respect to whether nucleocapsids and envelope glycoproteins travel toward axon termini separately or as assembled enveloped virions. Recently, a pseudorabies virus glycoprotein D (gD)-green fluorescent protein fusion was found to colocalize with viral capsids, supporting anterograde transport of enveloped virions. Previous antibody staining experiments demonstrated that herpes simplex virus (HSV) glycoproteins and capsids are separately transported in axons. Here, we generated an HSV expressing a gD-yellow fluorescent protein (YFP) fusion and found that gD-YFP and capsids were transported separately in neuronal axons. Anti-gD antibodies colocalized with gD-YFP, indicating that gD-YFP behaves like wild-type HSV gD.",
author = "Aleksandra Snyder and Birgitte Bruun and Browne, {Helena M.} and David Johnson",
year = "2007",
month = "8",
doi = "10.1128/JVI.00520-07",
language = "English (US)",
volume = "81",
pages = "8337--8340",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "15",

}

TY - JOUR

T1 - A herpes simplex virus gD-YFP fusion glycoprotein is transported separately from viral capsids in neuronal axons

AU - Snyder, Aleksandra

AU - Bruun, Birgitte

AU - Browne, Helena M.

AU - Johnson, David

PY - 2007/8

Y1 - 2007/8

N2 - Two models describing how alphaherpesviruses exit neurons differ with respect to whether nucleocapsids and envelope glycoproteins travel toward axon termini separately or as assembled enveloped virions. Recently, a pseudorabies virus glycoprotein D (gD)-green fluorescent protein fusion was found to colocalize with viral capsids, supporting anterograde transport of enveloped virions. Previous antibody staining experiments demonstrated that herpes simplex virus (HSV) glycoproteins and capsids are separately transported in axons. Here, we generated an HSV expressing a gD-yellow fluorescent protein (YFP) fusion and found that gD-YFP and capsids were transported separately in neuronal axons. Anti-gD antibodies colocalized with gD-YFP, indicating that gD-YFP behaves like wild-type HSV gD.

AB - Two models describing how alphaherpesviruses exit neurons differ with respect to whether nucleocapsids and envelope glycoproteins travel toward axon termini separately or as assembled enveloped virions. Recently, a pseudorabies virus glycoprotein D (gD)-green fluorescent protein fusion was found to colocalize with viral capsids, supporting anterograde transport of enveloped virions. Previous antibody staining experiments demonstrated that herpes simplex virus (HSV) glycoproteins and capsids are separately transported in axons. Here, we generated an HSV expressing a gD-yellow fluorescent protein (YFP) fusion and found that gD-YFP and capsids were transported separately in neuronal axons. Anti-gD antibodies colocalized with gD-YFP, indicating that gD-YFP behaves like wild-type HSV gD.

UR - http://www.scopus.com/inward/record.url?scp=34547133482&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547133482&partnerID=8YFLogxK

U2 - 10.1128/JVI.00520-07

DO - 10.1128/JVI.00520-07

M3 - Article

C2 - 17522199

AN - SCOPUS:34547133482

VL - 81

SP - 8337

EP - 8340

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 15

ER -