@article{8112b1c8903a44fc81eb770048dcf3cf,
title = "A global lipid map reveals host dependency factors conserved across SARS-CoV-2 variants",
abstract = "A comprehensive understanding of host dependency factors for SARS-CoV-2 remains elusive. Here, we map alterations in host lipids following SARS-CoV-2 infection using nontargeted lipidomics. We find that SARS-CoV-2 rewires host lipid metabolism, significantly altering hundreds of lipid species to effectively establish infection. We correlate these changes with viral protein activity by transfecting human cells with each viral protein and performing lipidomics. We find that lipid droplet plasticity is a key feature of infection and that viral propagation can be blocked by small-molecule glycerolipid biosynthesis inhibitors. We find that this inhibition was effective against the main variants of concern (alpha, beta, gamma, and delta), indicating that glycerolipid biosynthesis is a conserved host dependency factor that supports this evolving virus.",
author = "Farley, {Scotland E.} and Kyle, {Jennifer E.} and Leier, {Hans C.} and Bramer, {Lisa M.} and Weinstein, {Jules B.} and Bates, {Timothy A.} and Lee, {Joon Yong} and Metz, {Thomas O.} and Carsten Schultz and Tafesse, {Fikadu G.}",
note = "Funding Information: This work was supported by the National Institutes of Health (1RO1AI141549, received by FGT) and the Pulmonary & Critical Care training grant, “Multidisciplinary Research Training in Pulmonary Medicine” (T32HL083808, received by T.A.B.). Lipidomics analyses were performed in the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the Department of Energy (DOE) Office of Biological and Environmental Research located at the Pacific Northwest National Laboratory (PNNL). PNNL is a multiprogram national laboratory operated by Battelle for the DOE under Contract DE-AC05-76RLO 1830. J.E.K. and L.M.B. were supported by Laboratory Directed Research and Development Program at PNNL, and the National Institute of Environmental Health Sciences grant (U2CES030170, received by T.O.M.). PNNL is a multiprogram national laboratory operated by Battelle for the U.S. Department of Energy under contract DE-AC05-76RLO 1830. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1038/s41467-022-31097-7",
language = "English (US)",
volume = "13",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}