A gene that partially complements xeroderma pigmentosum group A cells maps to human chromosome 8

Gursurinder P. Kaur, Augustinus Rinaldy, R. Stephen Lloyd, Raghbir S. Athwal

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

A gene that partially complements sensitivity of xeroderma pigmentosum cells of group A to UV irradiation has been mapped to human chromosome 8. Isolation of this gene has previously been described. A cDNA clone pEMKR that represents part of this gene was used for mapping. Based upon the nucleotide sequence of pEMKR, a set of oligonucleotide primers were designed for PCR amplification of DNAs from hybrid cell lines. A panel of rodent-human hybrid cell lines representing the total human genome was screened by PCR and Southern blot analysis for chromosomal assignment of this gene. PCR amplification and hybridization occurred only in the case of human and hybrid cell lines that contained human chromosome 8. The pEMKR thus represents a different gene than a DNA repair gene XPAC that has been mapped to human chromosome 9.

Original languageEnglish (US)
Pages (from-to)371-379
Number of pages9
JournalSomatic Cell and Molecular Genetics
Volume18
Issue number4
DOIs
StatePublished - Jul 1 1992

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ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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