A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants

Ling Jie Kong, Beverly M. Orozco, Judith L. Roe, Steve Nagar, Sharon Ou, Heidi S. Feiler, Tim Durfee, Ann B. Miller, Wilhelm Gruissem, Dominique Robertson, Linda Hanley-Bowdoin

Research output: Contribution to journalArticle

153 Scopus citations

Abstract

Geminiviruses replicate in nuclei of mature plant cells after inducing the accumulation of host DNA replication machinery. Earlier studies showed that the viral replication factor, AL1, is sufficient for host induction and interacts with the cell cycle regulator, retinoblastoma (pRb). Unlike other DNA virus proteins, AL1 does not contain the pRb binding consensus, LXCXE, and interacts with plant pRb homologues (pRBR) through a novel amino acid sequence. We mapped the pRBR binding domain of AL1 between amino acids 101 and 180 and identified two mutants that are differentially impacted for AL1-pRBR interactions. Plants infected with the E-N140 mutant, which is wild-type for pRBR binding, developed wild-type symptoms and accumulated viral DNA and AL1 protein in epidermal, mesophyll and vascular cells of mature leaves. Plants inoculated with the KEE146 mutant, which retains 16% pRBR binding activity, only developed chlorosis along the veins, and viral DNA, AL1 protein and the host DNA synthesis factor, proliferating cell nuclear antigen, were localized to vascular tissue. These results established the importance of AL1-pRBR interactions during geminivirus infection of plants.

Original languageEnglish (US)
Pages (from-to)3485-3495
Number of pages11
JournalEMBO Journal
Volume19
Issue number13
DOIs
StatePublished - Jul 3 2000

Keywords

  • AL1
  • Cell cycle
  • Differentiation
  • Plant DNA virus
  • pRb

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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  • Cite this

    Kong, L. J., Orozco, B. M., Roe, J. L., Nagar, S., Ou, S., Feiler, H. S., Durfee, T., Miller, A. B., Gruissem, W., Robertson, D., & Hanley-Bowdoin, L. (2000). A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants. EMBO Journal, 19(13), 3485-3495. https://doi.org/10.1093/emboj/19.13.3485