A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants

Ling Jie Kong, Beverly M. Orozco, Judith L. Roe, Steve Nagar, Sharon Ou, Heidi Feiler, Tim Durfee, Ann B. Miller, Wilhelm Gruissem, Dominique Robertson, Linda Hanley-Bowdoin

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Geminiviruses replicate in nuclei of mature plant cells after inducing the accumulation of host DNA replication machinery. Earlier studies showed that the viral replication factor, AL1, is sufficient for host induction and interacts with the cell cycle regulator, retinoblastoma (pRb). Unlike other DNA virus proteins, AL1 does not contain the pRb binding consensus, LXCXE, and interacts with plant pRb homologues (pRBR) through a novel amino acid sequence. We mapped the pRBR binding domain of AL1 between amino acids 101 and 180 and identified two mutants that are differentially impacted for AL1-pRBR interactions. Plants infected with the E-N140 mutant, which is wild-type for pRBR binding, developed wild-type symptoms and accumulated viral DNA and AL1 protein in epidermal, mesophyll and vascular cells of mature leaves. Plants inoculated with the KEE146 mutant, which retains 16% pRBR binding activity, only developed chlorosis along the veins, and viral DNA, AL1 protein and the host DNA synthesis factor, proliferating cell nuclear antigen, were localized to vascular tissue. These results established the importance of AL1-pRBR interactions during geminivirus infection of plants.

Original languageEnglish (US)
Pages (from-to)3485-3495
Number of pages11
JournalEMBO Journal
Volume19
Issue number13
StatePublished - Jul 3 2000
Externally publishedYes

Fingerprint

Geminiviridae
Retinoblastoma Protein
Organ Specificity
Viral DNA
Tissue
Infection
Blood Vessels
Amino Acids
Proteins
Fibroblast Growth Factors
DNA
Proliferating Cell Nuclear Antigen
Mesophyll Cells
Hypochromic Anemia
Viruses
Machinery
DNA Viruses
Retinoblastoma
Plant Cells
DNA Replication

Keywords

  • AL1
  • Cell cycle
  • Differentiation
  • Plant DNA virus
  • pRb

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants. / Kong, Ling Jie; Orozco, Beverly M.; Roe, Judith L.; Nagar, Steve; Ou, Sharon; Feiler, Heidi; Durfee, Tim; Miller, Ann B.; Gruissem, Wilhelm; Robertson, Dominique; Hanley-Bowdoin, Linda.

In: EMBO Journal, Vol. 19, No. 13, 03.07.2000, p. 3485-3495.

Research output: Contribution to journalArticle

Kong, LJ, Orozco, BM, Roe, JL, Nagar, S, Ou, S, Feiler, H, Durfee, T, Miller, AB, Gruissem, W, Robertson, D & Hanley-Bowdoin, L 2000, 'A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants', EMBO Journal, vol. 19, no. 13, pp. 3485-3495.
Kong, Ling Jie ; Orozco, Beverly M. ; Roe, Judith L. ; Nagar, Steve ; Ou, Sharon ; Feiler, Heidi ; Durfee, Tim ; Miller, Ann B. ; Gruissem, Wilhelm ; Robertson, Dominique ; Hanley-Bowdoin, Linda. / A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants. In: EMBO Journal. 2000 ; Vol. 19, No. 13. pp. 3485-3495.
@article{25baf97b3d2b4369b8ce689e93004e12,
title = "A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants",
abstract = "Geminiviruses replicate in nuclei of mature plant cells after inducing the accumulation of host DNA replication machinery. Earlier studies showed that the viral replication factor, AL1, is sufficient for host induction and interacts with the cell cycle regulator, retinoblastoma (pRb). Unlike other DNA virus proteins, AL1 does not contain the pRb binding consensus, LXCXE, and interacts with plant pRb homologues (pRBR) through a novel amino acid sequence. We mapped the pRBR binding domain of AL1 between amino acids 101 and 180 and identified two mutants that are differentially impacted for AL1-pRBR interactions. Plants infected with the E-N140 mutant, which is wild-type for pRBR binding, developed wild-type symptoms and accumulated viral DNA and AL1 protein in epidermal, mesophyll and vascular cells of mature leaves. Plants inoculated with the KEE146 mutant, which retains 16{\%} pRBR binding activity, only developed chlorosis along the veins, and viral DNA, AL1 protein and the host DNA synthesis factor, proliferating cell nuclear antigen, were localized to vascular tissue. These results established the importance of AL1-pRBR interactions during geminivirus infection of plants.",
keywords = "AL1, Cell cycle, Differentiation, Plant DNA virus, pRb",
author = "Kong, {Ling Jie} and Orozco, {Beverly M.} and Roe, {Judith L.} and Steve Nagar and Sharon Ou and Heidi Feiler and Tim Durfee and Miller, {Ann B.} and Wilhelm Gruissem and Dominique Robertson and Linda Hanley-Bowdoin",
year = "2000",
month = "7",
day = "3",
language = "English (US)",
volume = "19",
pages = "3485--3495",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "13",

}

TY - JOUR

T1 - A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants

AU - Kong, Ling Jie

AU - Orozco, Beverly M.

AU - Roe, Judith L.

AU - Nagar, Steve

AU - Ou, Sharon

AU - Feiler, Heidi

AU - Durfee, Tim

AU - Miller, Ann B.

AU - Gruissem, Wilhelm

AU - Robertson, Dominique

AU - Hanley-Bowdoin, Linda

PY - 2000/7/3

Y1 - 2000/7/3

N2 - Geminiviruses replicate in nuclei of mature plant cells after inducing the accumulation of host DNA replication machinery. Earlier studies showed that the viral replication factor, AL1, is sufficient for host induction and interacts with the cell cycle regulator, retinoblastoma (pRb). Unlike other DNA virus proteins, AL1 does not contain the pRb binding consensus, LXCXE, and interacts with plant pRb homologues (pRBR) through a novel amino acid sequence. We mapped the pRBR binding domain of AL1 between amino acids 101 and 180 and identified two mutants that are differentially impacted for AL1-pRBR interactions. Plants infected with the E-N140 mutant, which is wild-type for pRBR binding, developed wild-type symptoms and accumulated viral DNA and AL1 protein in epidermal, mesophyll and vascular cells of mature leaves. Plants inoculated with the KEE146 mutant, which retains 16% pRBR binding activity, only developed chlorosis along the veins, and viral DNA, AL1 protein and the host DNA synthesis factor, proliferating cell nuclear antigen, were localized to vascular tissue. These results established the importance of AL1-pRBR interactions during geminivirus infection of plants.

AB - Geminiviruses replicate in nuclei of mature plant cells after inducing the accumulation of host DNA replication machinery. Earlier studies showed that the viral replication factor, AL1, is sufficient for host induction and interacts with the cell cycle regulator, retinoblastoma (pRb). Unlike other DNA virus proteins, AL1 does not contain the pRb binding consensus, LXCXE, and interacts with plant pRb homologues (pRBR) through a novel amino acid sequence. We mapped the pRBR binding domain of AL1 between amino acids 101 and 180 and identified two mutants that are differentially impacted for AL1-pRBR interactions. Plants infected with the E-N140 mutant, which is wild-type for pRBR binding, developed wild-type symptoms and accumulated viral DNA and AL1 protein in epidermal, mesophyll and vascular cells of mature leaves. Plants inoculated with the KEE146 mutant, which retains 16% pRBR binding activity, only developed chlorosis along the veins, and viral DNA, AL1 protein and the host DNA synthesis factor, proliferating cell nuclear antigen, were localized to vascular tissue. These results established the importance of AL1-pRBR interactions during geminivirus infection of plants.

KW - AL1

KW - Cell cycle

KW - Differentiation

KW - Plant DNA virus

KW - pRb

UR - http://www.scopus.com/inward/record.url?scp=0034600840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034600840&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 3485

EP - 3495

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 13

ER -