A frameshift at a mutational hotspot in the polyoma virus early region generates two new proteins that define T-antigen functional domains

Joanna B. Wilson, Adrian Hayday, Sara Courtneidge, Mike Fried

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

A frameshift mutation, arising from the deletion of any one of nine consecutive cytidines in the region of Py DNA encoding both the midregion of large T-Ag and the C-terminal region of middle T-Ag, yields unstable flat cell revertants that synthesize two novel viral proteins in which shuffling of the different domains of the Py T-Ags has occurred. The first protein (37 kd) is a hybrid containing the N-terminus of large T-Ag and the hydrophobic C-terminus of middle T-Ag. The latter domain is responsible for membrane association, even in the 37 kd hybrid protein. The second protein (43 kd), which contains the N-terminal 75% of middle T-Ag, has an associated protein kinase activity and forms a complex with c-src, but cannot induce a transformed phenotype.

Original languageEnglish (US)
Pages (from-to)477-487
Number of pages11
JournalCell
Volume44
Issue number3
DOIs
StatePublished - Feb 14 1986

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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