A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening

Motoyuki Hattori, Ryan E. Hibbs, Eric Gouaux

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Optimization of membrane protein stability under different solution conditions is essential for obtaining crystals that diffract to high resolution. Traditional methods that evaluate protein stability require large amounts of material and are, therefore, ill suited for medium- to high-throughput screening of membrane proteins. Here we present a rapid and efficient fluorescence-detection size-exclusion chromatography-based thermostability assay (FSEC-TS). In this method, the target protein is fused to GFP. Heated protein samples, treated with a panel of additives, are then analyzed by FSEC. FSEC-TS allows one to evaluate the thermostability of nanogram-to-microgram amounts of the target protein under a variety of conditions without purification. We applied this method to the Danio rerio P2X4 receptor and Caenorhabditis elegans GluCl to screen ligands, ions, and lipids, including newly designed cholesterol derivatives. In the case of GluCl, the screening results were used to obtain crystals of the receptor in the presence of lipids.

Original languageEnglish (US)
Pages (from-to)1293-1299
Number of pages7
JournalStructure
Volume20
Issue number8
DOIs
StatePublished - Aug 8 2012

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Gel Chromatography
Membrane Proteins
Protein Stability
Fluorescence
Purinergic P2X4 Receptors
Lipids
Proteins
Caenorhabditis elegans
Zebrafish
Cholesterol
Ions
Ligands

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening. / Hattori, Motoyuki; Hibbs, Ryan E.; Gouaux, Eric.

In: Structure, Vol. 20, No. 8, 08.08.2012, p. 1293-1299.

Research output: Contribution to journalArticle

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