A flow system for partial automation of plating efficiency tests

S. Macevicz, P. Dean, D. Peters, D. Pinkel, J. Gray

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A flow system that sterilely deposits a known number of cells into a collection vessel has been constructed and evaluated. In this device, the cells from a single‐cell suspension flow through a nozzle that constrains them to the center of the emerging jet. The cells in the jet are illuminated by a 0.5‐mW helium‐neon laser; they are detected, sized and counted on the basis of the light scattered as they pass through the laser beam. The device has been used for plating efficiency tests in a tissue culture laboratory. We found that it saved considerable labor by eliminating the dilution step from the standard plating efficiency procedure. The biologic effect of machine plating was determined by comparing plating efficiencies of KHT, CHO‐T5, and CHO‐AA8 cells established by hand plating with those established by machine plating. Results indicate that machine plating reduces the plating efficiency of the CHO‐T5 cells while the plating efficiencies of the CHO‐AA8 and KHT cells are unaffected.

Original languageEnglish (US)
Pages (from-to)346-350
Number of pages5
JournalCytometry
Volume1
Issue number5
DOIs
StatePublished - Mar 1981

Keywords

  • Flow system
  • cell counting
  • detrimental effects
  • plating efficiency tests

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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