A fate map of chick otic cup closure reveals lineage boundaries in the dorsal otocyst

John Brigande, Laurie E. Iten, Donna M. Fekete

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The vertebrate inner ear is structurally complex, consisting of fluid-filled tubules and sensory organs that subserve the functions of hearing and balance. The epithelial parts of the inner ear are derived from the otic placode, which deepens to form a cup before closing to form the otic vesicle. We fate-mapped the rim of the otic cup to monitor the cellular movements associated with otocyst formation and to aid in interpreting the changing gene expression patterns of the early otic field. Twelve sites around the rim, defined as positions of a clock face, were targeted by iontophoretic injection of fluorescent, lipophilic dye. Labeled cells were imaged 24 and 48 h after injection. The data show that the entire dorsal rim of the otic cup becomes the endolymphatic duct (ED), while the posteroventral rim becomes the lateral otocyst wall. Two intersecting boundaries of lineage restriction were identified near the dorsal pole: one bisecting the ED into anterior and posterior halves and the other defining its lateral edge. We hypothesize that signaling across compartment boundaries may play a critical role in duct specification. This model is discussed in the context of mouse mutants that are defective in both hindbrain development and ED outgrowth. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)256-270
Number of pages15
JournalDevelopmental Biology
Volume227
Issue number2
DOIs
StatePublished - Nov 15 2000
Externally publishedYes

Fingerprint

Endolymphatic Duct
Ear
Inner Ear
Rhombencephalon
Injections
Fluorescent Dyes
Hearing
Vertebrates
Gene Expression

Keywords

  • Endolymphatic duct
  • Fate map
  • Inner ear
  • Lineage boundary
  • Otic cup

ASJC Scopus subject areas

  • Developmental Biology

Cite this

A fate map of chick otic cup closure reveals lineage boundaries in the dorsal otocyst. / Brigande, John; Iten, Laurie E.; Fekete, Donna M.

In: Developmental Biology, Vol. 227, No. 2, 15.11.2000, p. 256-270.

Research output: Contribution to journalArticle

Brigande, John ; Iten, Laurie E. ; Fekete, Donna M. / A fate map of chick otic cup closure reveals lineage boundaries in the dorsal otocyst. In: Developmental Biology. 2000 ; Vol. 227, No. 2. pp. 256-270.
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