A dual parameter FRET probe for measuring PKC and PKA activity in living cells

Justin Brumbaugh, Andreas Schleifenbaum, Alexander Gasch, Michael Sattler, Carsten Schultz

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Cell function is regulated by complex and often interdependent networks of signaling molecules. To accurately describe these networks, it is important to monitor multiple signals in parallel. To this end, we have developed a genetically encoded, FRET-based probe that independently monitors both protein kinase A (PKA) and protein kinase C (PKC) activity in vivo. Artificial as well as physiological stimulants produced a negative or positive change in FRET efficiency following PKA or PKC activation, respectively. Mutations of the phosphate-accepting amino acids of the PKC substrate yielded a probe that was sensitive to PKA activation alone.

Original languageEnglish (US)
Pages (from-to)24-25
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number1
DOIs
StatePublished - Jan 11 2006

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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