A Drosophila KCNQ channel essential for early embryonic development

Hua Wen, Thomas M. Weiger, Tanya S. Ferguson, Mohammad Shahidullah, Samae S. Scott, Irwin B. Levitan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The mammalian voltage-dependent KCNQ channels are responsible for distinct types of native potassium currents and are associated with several human diseases. We cloned a novel Drosophila KCNQ channel (dKCNQ) based on its sequence homology to the mammalian genes. When expressed in Chinese hamster ovary cells, dKCNQ gives rise to a slowly activating and slowly deactivating current that activates in the subthreshold voltage range. Like the M-current produced by mammalian KCNQ channels, dKCNQ current is sensitive to the KCNQ-specific blocker linopirdine and is suppressed by activation of a muscarinic receptor. dKCNQ is also similar to the mammalian channels in that it binds calmodulin (CaM), and CaM binding is necessary to produce functional currents. In situ hybridization analysis demonstrates that dKCNQ mRNA is present in brain cortical neurons, the cardia (proventriculus), and the nurse cells and oocytes of the ovary. We generated mutant flies with deletions in the genomic sequence of dKCNQ. Embryos produced by homozygous deletion females exhibit disorganized nuclei and fail to hatch, suggesting strongly that a maternal contribution of dKCNQ protein and/or mRNA is essential for early embryonic development.

Original languageEnglish (US)
Pages (from-to)10147-10156
Number of pages10
JournalJournal of Neuroscience
Volume25
Issue number44
DOIs
StatePublished - Nov 2 2005
Externally publishedYes

Fingerprint

Drosophila
Embryonic Development
linopirdine
Calmodulin
Ovary
Proventriculus
Messenger RNA
Cardia
Muscarinic Receptors
Sequence Homology
Cricetulus
Diptera
Oocytes
In Situ Hybridization
Potassium
Embryonic Structures
Nurses
Mothers
Neurons
Brain

Keywords

  • Drosophila ion channels
  • Embryonic development
  • Fly mutants
  • KCNQ channels
  • M-current
  • Maternal effect

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Wen, H., Weiger, T. M., Ferguson, T. S., Shahidullah, M., Scott, S. S., & Levitan, I. B. (2005). A Drosophila KCNQ channel essential for early embryonic development. Journal of Neuroscience, 25(44), 10147-10156. https://doi.org/10.1523/JNEUROSCI.3086-05.2005

A Drosophila KCNQ channel essential for early embryonic development. / Wen, Hua; Weiger, Thomas M.; Ferguson, Tanya S.; Shahidullah, Mohammad; Scott, Samae S.; Levitan, Irwin B.

In: Journal of Neuroscience, Vol. 25, No. 44, 02.11.2005, p. 10147-10156.

Research output: Contribution to journalArticle

Wen, H, Weiger, TM, Ferguson, TS, Shahidullah, M, Scott, SS & Levitan, IB 2005, 'A Drosophila KCNQ channel essential for early embryonic development', Journal of Neuroscience, vol. 25, no. 44, pp. 10147-10156. https://doi.org/10.1523/JNEUROSCI.3086-05.2005
Wen H, Weiger TM, Ferguson TS, Shahidullah M, Scott SS, Levitan IB. A Drosophila KCNQ channel essential for early embryonic development. Journal of Neuroscience. 2005 Nov 2;25(44):10147-10156. https://doi.org/10.1523/JNEUROSCI.3086-05.2005
Wen, Hua ; Weiger, Thomas M. ; Ferguson, Tanya S. ; Shahidullah, Mohammad ; Scott, Samae S. ; Levitan, Irwin B. / A Drosophila KCNQ channel essential for early embryonic development. In: Journal of Neuroscience. 2005 ; Vol. 25, No. 44. pp. 10147-10156.
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