A Drosophila G-protein a subunit, Gfα, expressed in a spatially and temporally restricted pattern during Drosophila development

Franklin Quan, William J. Wolfgang, Michael Forte

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24 Citations (Scopus)

Abstract

Heterotrimeric guanine nucleotide binding proteins (G proteins) couple receptors for extracellular signals to intracellular second messenger-generating systems. Previous studies have shown that G-protein α subunits (Gα) are expressed in a spatially and temporally restricted manner during Drosophila embryogenesis and, thus, may be responsible for mediating developmental interactions. We have used the polymerase chain reaction to search for specific Gα subunits that function primarily during development and not in the adult fly. Using poly(A)+ RNA isolated from early pupae (day 1), we have isolated a cDNA coding for a fly Gα subunit, designated Gfα. This subunit is 30-38% identical to previously described vertebrate and Drosophila Gα subunits and appears to define an additional family of Gα proteins. Gfα transcripts are expressed primarily during embryonic, larval, and early pupal stages and only at low levels in adult flies. In situ hybridization studies indicate that Gfα transcripts are expressed maternally and later during embryogenesis primarily in the developing midgut and transiently in the amnioserosa. The Gfα gene has been characterized and mapped to position 73B of the Drosophila genome.

Original languageEnglish (US)
Pages (from-to)4236-4240
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number9
StatePublished - May 1 1993

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Protein Subunits
GTP-Binding Proteins
Diptera
Drosophila
Second Messenger Systems
Embryonic Development
Pupa
Guanine Nucleotides
In Situ Hybridization
Vertebrates
Carrier Proteins
Complementary DNA
Genome
Polymerase Chain Reaction
Messenger RNA
Genes
Drosophila garnet protein

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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abstract = "Heterotrimeric guanine nucleotide binding proteins (G proteins) couple receptors for extracellular signals to intracellular second messenger-generating systems. Previous studies have shown that G-protein α subunits (Gα) are expressed in a spatially and temporally restricted manner during Drosophila embryogenesis and, thus, may be responsible for mediating developmental interactions. We have used the polymerase chain reaction to search for specific Gα subunits that function primarily during development and not in the adult fly. Using poly(A)+ RNA isolated from early pupae (day 1), we have isolated a cDNA coding for a fly Gα subunit, designated Gfα. This subunit is 30-38{\%} identical to previously described vertebrate and Drosophila Gα subunits and appears to define an additional family of Gα proteins. Gfα transcripts are expressed primarily during embryonic, larval, and early pupal stages and only at low levels in adult flies. In situ hybridization studies indicate that Gfα transcripts are expressed maternally and later during embryogenesis primarily in the developing midgut and transiently in the amnioserosa. The Gfα gene has been characterized and mapped to position 73B of the Drosophila genome.",
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T1 - A Drosophila G-protein a subunit, Gfα, expressed in a spatially and temporally restricted pattern during Drosophila development

AU - Quan, Franklin

AU - Wolfgang, William J.

AU - Forte, Michael

PY - 1993/5/1

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N2 - Heterotrimeric guanine nucleotide binding proteins (G proteins) couple receptors for extracellular signals to intracellular second messenger-generating systems. Previous studies have shown that G-protein α subunits (Gα) are expressed in a spatially and temporally restricted manner during Drosophila embryogenesis and, thus, may be responsible for mediating developmental interactions. We have used the polymerase chain reaction to search for specific Gα subunits that function primarily during development and not in the adult fly. Using poly(A)+ RNA isolated from early pupae (day 1), we have isolated a cDNA coding for a fly Gα subunit, designated Gfα. This subunit is 30-38% identical to previously described vertebrate and Drosophila Gα subunits and appears to define an additional family of Gα proteins. Gfα transcripts are expressed primarily during embryonic, larval, and early pupal stages and only at low levels in adult flies. In situ hybridization studies indicate that Gfα transcripts are expressed maternally and later during embryogenesis primarily in the developing midgut and transiently in the amnioserosa. The Gfα gene has been characterized and mapped to position 73B of the Drosophila genome.

AB - Heterotrimeric guanine nucleotide binding proteins (G proteins) couple receptors for extracellular signals to intracellular second messenger-generating systems. Previous studies have shown that G-protein α subunits (Gα) are expressed in a spatially and temporally restricted manner during Drosophila embryogenesis and, thus, may be responsible for mediating developmental interactions. We have used the polymerase chain reaction to search for specific Gα subunits that function primarily during development and not in the adult fly. Using poly(A)+ RNA isolated from early pupae (day 1), we have isolated a cDNA coding for a fly Gα subunit, designated Gfα. This subunit is 30-38% identical to previously described vertebrate and Drosophila Gα subunits and appears to define an additional family of Gα proteins. Gfα transcripts are expressed primarily during embryonic, larval, and early pupal stages and only at low levels in adult flies. In situ hybridization studies indicate that Gfα transcripts are expressed maternally and later during embryogenesis primarily in the developing midgut and transiently in the amnioserosa. The Gfα gene has been characterized and mapped to position 73B of the Drosophila genome.

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