A distinguishing gene signature shared by tumor-infiltrating Tie2-expressing monocytes, blood "resident" monocytes, and embryonic macrophages suggests common functions and developmental relationships

Ferdinando Pucci, Mary Anna Venneri, Daniela Biziato, Alessandro Nonis, Davide Moi, Antonio Sica, Clelia Di Serio, Luigi Naldini, Michele De Palma

Research output: Contribution to journalArticle

239 Citations (Scopus)

Abstract

We previously showed that Tie2-expressing monocytes (TEMs) have nonredundant proangiogenic activity in tumors. Here, we compared the gene expression profile of tumor-infiltrating TEMs with that of tumor-associated macrophages (TAMs), spleen-derived Gr1+Cd11b+ neutrophils/myeloid-derived suppressor cells, circulating " inflammatory" and "resident" monocytes, and tumor-derived endothelial cells (ECs) by quantitative polymerase chain reaction-based gene arrays. TEMs sharply differed from ECs and Gr1+Cd11b+ cells but were highly related to TAMs. Nevertheless, several genes were differentially expressed between TEMs and TAMs, highlighting a TEM signature consistent with enhanced proangiogenic/tissue-remodeling activity and lower proinflammatory activity. We validated these findings in models of oncogenesis and transgenic mice expressing a microRNA-regulated Tie2-GFP reporter. Remarkably, resident monocytes and TEMs on one hand, and inflammatory monocytes and TAMs on the other hand, expressed coordinated gene expression profiles, suggesting that the 2 blood monocyte subsets are committed to distinct extravascular fates in the tumor microenvironment. We further showed that a prominent proportion of embryonic/fetal macrophages, which participate in tissue morphogenesis, expressed distinguishing TEM genes. It is tempting to speculate that Tie2+ embryonic/fetal macrophages, resident blood monocytes, and tumor-infiltrating TEMs represent distinct developmental stages of a TEM lineage committed to execute physiologic proangiogenic and tissue-remodeling programs, which can be coopted by tumors.

Original languageEnglish (US)
Pages (from-to)901-914
Number of pages14
JournalBlood
Volume114
Issue number4
DOIs
StatePublished - Nov 19 2009
Externally publishedYes

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Macrophages
Tumors
Monocytes
Blood
Genes
Neoplasms
Endothelial cells
Tissue
Gene expression
Transcriptome
Polymerase chain reaction
Endothelial Cells
MicroRNAs
Tumor Microenvironment
Morphogenesis
Transgenic Mice
Carcinogenesis
Neutrophils

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

A distinguishing gene signature shared by tumor-infiltrating Tie2-expressing monocytes, blood "resident" monocytes, and embryonic macrophages suggests common functions and developmental relationships. / Pucci, Ferdinando; Venneri, Mary Anna; Biziato, Daniela; Nonis, Alessandro; Moi, Davide; Sica, Antonio; Di Serio, Clelia; Naldini, Luigi; De Palma, Michele.

In: Blood, Vol. 114, No. 4, 19.11.2009, p. 901-914.

Research output: Contribution to journalArticle

Pucci, Ferdinando ; Venneri, Mary Anna ; Biziato, Daniela ; Nonis, Alessandro ; Moi, Davide ; Sica, Antonio ; Di Serio, Clelia ; Naldini, Luigi ; De Palma, Michele. / A distinguishing gene signature shared by tumor-infiltrating Tie2-expressing monocytes, blood "resident" monocytes, and embryonic macrophages suggests common functions and developmental relationships. In: Blood. 2009 ; Vol. 114, No. 4. pp. 901-914.
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AU - Biziato, Daniela

AU - Nonis, Alessandro

AU - Moi, Davide

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