A direct method for the calculation of alloreactive CD4+ T cell precursor frequency

Hooman Noorchashm, Yen K. Lieu, Susan Y. Rostami, Howard Song, Siri A S Greeley, Samaneh Bazel, Clyde F. Barker, Ali Naji

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background. Direct measurement of the precursor frequency of alloreactive CD4+ T cells has been impossible due to the lack of a specific means of determining the absolute number of daughter cells generated with each division in a repertoire of stimulated T cells. Methods. Responder lymphocytes were fluorescently labeled and adoptively transferred into irradiated allogeneic stimulator mice or incubated in vitro with irradiated stimulator splenocytes. After a 65- to 70-hr stimulation period, responder cells were analyzed by flow cytometry. Results. The precursor frequency of dividing CD4+ T cells was determined both in vive and in vitro. The observed number of alloreactive daughter cells generated with each round of division was used to calculate the frequency of alloantigen-specific CD4+ T cells. Conclusions. A novel method for the direct calculation of the frequency of alloreactive CD4+ T cells is described. This technique allows the determination of changes in the frequency of alloreactive T cells that might underlie tolerance to alloantigens.

Original languageEnglish (US)
Pages (from-to)1281-1284
Number of pages4
JournalTransplantation
Volume67
Issue number9
DOIs
StatePublished - May 15 1999
Externally publishedYes

Fingerprint

T-Lymphoid Precursor Cells
T-Lymphocytes
Isoantigens
Flow Cytometry
Cell Count
Lymphocytes

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Noorchashm, H., Lieu, Y. K., Rostami, S. Y., Song, H., Greeley, S. A. S., Bazel, S., ... Naji, A. (1999). A direct method for the calculation of alloreactive CD4+ T cell precursor frequency. Transplantation, 67(9), 1281-1284. https://doi.org/10.1097/00007890-199905150-00015

A direct method for the calculation of alloreactive CD4+ T cell precursor frequency. / Noorchashm, Hooman; Lieu, Yen K.; Rostami, Susan Y.; Song, Howard; Greeley, Siri A S; Bazel, Samaneh; Barker, Clyde F.; Naji, Ali.

In: Transplantation, Vol. 67, No. 9, 15.05.1999, p. 1281-1284.

Research output: Contribution to journalArticle

Noorchashm, H, Lieu, YK, Rostami, SY, Song, H, Greeley, SAS, Bazel, S, Barker, CF & Naji, A 1999, 'A direct method for the calculation of alloreactive CD4+ T cell precursor frequency', Transplantation, vol. 67, no. 9, pp. 1281-1284. https://doi.org/10.1097/00007890-199905150-00015
Noorchashm, Hooman ; Lieu, Yen K. ; Rostami, Susan Y. ; Song, Howard ; Greeley, Siri A S ; Bazel, Samaneh ; Barker, Clyde F. ; Naji, Ali. / A direct method for the calculation of alloreactive CD4+ T cell precursor frequency. In: Transplantation. 1999 ; Vol. 67, No. 9. pp. 1281-1284.
@article{6c52fc06842047d49bfba3aef4ce2276,
title = "A direct method for the calculation of alloreactive CD4+ T cell precursor frequency",
abstract = "Background. Direct measurement of the precursor frequency of alloreactive CD4+ T cells has been impossible due to the lack of a specific means of determining the absolute number of daughter cells generated with each division in a repertoire of stimulated T cells. Methods. Responder lymphocytes were fluorescently labeled and adoptively transferred into irradiated allogeneic stimulator mice or incubated in vitro with irradiated stimulator splenocytes. After a 65- to 70-hr stimulation period, responder cells were analyzed by flow cytometry. Results. The precursor frequency of dividing CD4+ T cells was determined both in vive and in vitro. The observed number of alloreactive daughter cells generated with each round of division was used to calculate the frequency of alloantigen-specific CD4+ T cells. Conclusions. A novel method for the direct calculation of the frequency of alloreactive CD4+ T cells is described. This technique allows the determination of changes in the frequency of alloreactive T cells that might underlie tolerance to alloantigens.",
author = "Hooman Noorchashm and Lieu, {Yen K.} and Rostami, {Susan Y.} and Howard Song and Greeley, {Siri A S} and Samaneh Bazel and Barker, {Clyde F.} and Ali Naji",
year = "1999",
month = "5",
day = "15",
doi = "10.1097/00007890-199905150-00015",
language = "English (US)",
volume = "67",
pages = "1281--1284",
journal = "Transplantation",
issn = "0041-1337",
publisher = "Lippincott Williams and Wilkins",
number = "9",

}

TY - JOUR

T1 - A direct method for the calculation of alloreactive CD4+ T cell precursor frequency

AU - Noorchashm, Hooman

AU - Lieu, Yen K.

AU - Rostami, Susan Y.

AU - Song, Howard

AU - Greeley, Siri A S

AU - Bazel, Samaneh

AU - Barker, Clyde F.

AU - Naji, Ali

PY - 1999/5/15

Y1 - 1999/5/15

N2 - Background. Direct measurement of the precursor frequency of alloreactive CD4+ T cells has been impossible due to the lack of a specific means of determining the absolute number of daughter cells generated with each division in a repertoire of stimulated T cells. Methods. Responder lymphocytes were fluorescently labeled and adoptively transferred into irradiated allogeneic stimulator mice or incubated in vitro with irradiated stimulator splenocytes. After a 65- to 70-hr stimulation period, responder cells were analyzed by flow cytometry. Results. The precursor frequency of dividing CD4+ T cells was determined both in vive and in vitro. The observed number of alloreactive daughter cells generated with each round of division was used to calculate the frequency of alloantigen-specific CD4+ T cells. Conclusions. A novel method for the direct calculation of the frequency of alloreactive CD4+ T cells is described. This technique allows the determination of changes in the frequency of alloreactive T cells that might underlie tolerance to alloantigens.

AB - Background. Direct measurement of the precursor frequency of alloreactive CD4+ T cells has been impossible due to the lack of a specific means of determining the absolute number of daughter cells generated with each division in a repertoire of stimulated T cells. Methods. Responder lymphocytes were fluorescently labeled and adoptively transferred into irradiated allogeneic stimulator mice or incubated in vitro with irradiated stimulator splenocytes. After a 65- to 70-hr stimulation period, responder cells were analyzed by flow cytometry. Results. The precursor frequency of dividing CD4+ T cells was determined both in vive and in vitro. The observed number of alloreactive daughter cells generated with each round of division was used to calculate the frequency of alloantigen-specific CD4+ T cells. Conclusions. A novel method for the direct calculation of the frequency of alloreactive CD4+ T cells is described. This technique allows the determination of changes in the frequency of alloreactive T cells that might underlie tolerance to alloantigens.

UR - http://www.scopus.com/inward/record.url?scp=0033562750&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033562750&partnerID=8YFLogxK

U2 - 10.1097/00007890-199905150-00015

DO - 10.1097/00007890-199905150-00015

M3 - Article

VL - 67

SP - 1281

EP - 1284

JO - Transplantation

JF - Transplantation

SN - 0041-1337

IS - 9

ER -