A dentin-derived hydrogel bioink for 3D bioprinting of cell laden scaffolds for regenerative dentistry

Avathamsa Athirasala, Anthony Tahayeri, Greeshma Thrivikraman, Cristiane M. Franca, Nelson Monteiro, Victor Tran, Jack Ferracane, Luiz Bertassoni

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Recent studies in tissue engineering have adopted extracellular matrix (ECM) derived scaffolds as natural and cytocompatible microenvironments for tissue regeneration. The dentin matrix, specifically, has been shown to be associated with a host of soluble and insoluble signaling molecules that can promote odontogenesis. Here, we have developed a novel bioink, blending printable alginate (3% w/v) hydrogels with the soluble and insoluble fractions of the dentin matrix. We have optimized the printing parameters and the concentrations of the individual components of the bioink for print accuracy, cell viability and odontogenic potential. We find that, while viscosity, and hence printability of the bioinks, was greater in the formulations containing higher concentrations of alginate, a higher proportion of insoluble dentin matrix proteins significantly improved cell viability; where a 1:1 ratio of alginate and dentin (1:1 Alg-Dent) was most suitable. We further demonstrate high retention of the soluble dentin molecules within the 1:1 Alg-Dent hydrogel blends, evidencing renewed interactions between these molecules and the dentin matrix post crosslinking. Moreover, at concentrations of 100 μg ml-1, these soluble dentin molecules significantly enhanced odontogenic differentiation of stem cells from the apical papilla encapsulated in bioprinted hydrogels. In summary, the proposed novel bioinks have demonstrable cytocompatibility and natural odontogenic capacity, which can be a used to reproducibly fabricate scaffolds with complex three-dimensional microarchitectures for regenerative dentistry in the future.

Original languageEnglish (US)
Article number024101
JournalBiofabrication
Volume10
Issue number2
DOIs
StatePublished - Jan 10 2018

Fingerprint

Bioprinting
Dentistry
Hydrogel
Dentin
Scaffolds (biology)
Hydrogels
Alginate
Molecules
Scaffolds
Cells
Tissue regeneration
Stem cells
Tissue engineering
Cell Survival
Crosslinking
Odontogenesis
Printing
Viscosity
Proteins
Tissue Engineering

Keywords

  • 3D printing
  • alginate
  • bioprinting
  • dentin
  • hydrogel bioink
  • pulp regeneration
  • regenerative dentistry

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

A dentin-derived hydrogel bioink for 3D bioprinting of cell laden scaffolds for regenerative dentistry. / Athirasala, Avathamsa; Tahayeri, Anthony; Thrivikraman, Greeshma; Franca, Cristiane M.; Monteiro, Nelson; Tran, Victor; Ferracane, Jack; Bertassoni, Luiz.

In: Biofabrication, Vol. 10, No. 2, 024101, 10.01.2018.

Research output: Contribution to journalArticle

Athirasala A, Tahayeri A, Thrivikraman G, Franca CM, Monteiro N, Tran V et al. A dentin-derived hydrogel bioink for 3D bioprinting of cell laden scaffolds for regenerative dentistry. Biofabrication. 2018 Jan 10;10(2). 024101. https://doi.org/10.1088/1758-5090/aa9b4e
Athirasala, Avathamsa ; Tahayeri, Anthony ; Thrivikraman, Greeshma ; Franca, Cristiane M. ; Monteiro, Nelson ; Tran, Victor ; Ferracane, Jack ; Bertassoni, Luiz. / A dentin-derived hydrogel bioink for 3D bioprinting of cell laden scaffolds for regenerative dentistry. In: Biofabrication. 2018 ; Vol. 10, No. 2.
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