@article{cf2920ffb6ee44a5969b05ceac3243eb,
title = "A cytoskeletal protein complex is essential for division of intracellular amastigotes of Leishmania mexicana",
abstract = "Previous studies in Leishmania mexicana have identified the cytoskeletal protein KHARON as being important for both flagellar trafficking of the glucose transporter GT1 and for successful cytokinesis and survival of infectious amastigote forms inside mammalian macrophages. KHARON is located in three distinct regions of the cytoskeleton: the base of the flagellum, the subpellicular microtubules, and the mitotic spindle. To deconvolve the different functions for KHARON, we have identified two partner proteins, KHAP1 and KHAP2, which associate with KHARON. KHAP1 is located only in the subpellicular microtubules, whereas KHAP2 is located at the subpellicular microtubules and the base of the flagellum. Both KHAP1 and KHAP2 null mutants are unable to execute cytokinesis but are able to traffic GT1 to the flagellum. These results confirm that KHARON assembles into distinct functional complexes and that the subpellicular complex is essential for cytokinesis and viability of disease-causing amastigotes but not for flagellar membrane trafficking.",
author = "Kelly, {Felice D.} and Tran, {Khoa D.} and Jess Hatfield and Kat Schmidt and Sanchez, {Marco A.} and Landfear, {Scott M.}",
note = "Funding Information: Funding and additional information—This work was supported by National Institutes of Health Grants R01AI121160 to F. D. K., J. H., K. S., M. A. S., and S. M. L. and R21AI107144 (to F. D. K. and S. M. L.) and 1F32AI096854 (to K. D. T.). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Funding Information: Acknowledgments—Mass spectrometric analysis was performed by the OHSU Proteomics Shared Resource with partial support from National Institutes of Health core Grants P30EY010572 and P30CA069533. We acknowledge its director Larry David for performing the proteomics experiments and Phillip Wilmarth for bio-informatic analysis of the proteomics data. We appreciate the expert advice and support of the staff of the Advanced Light Microscopy Core in the Jungers Center for Neurosciences at Oregon Health & Science University. Publisher Copyright: {\textcopyright} 2020 Kelly et al. Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.",
year = "2020",
month = sep,
day = "11",
doi = "10.1074/jbc.ra120.014065",
language = "English (US)",
volume = "295",
pages = "13106--13122",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "37",
}