A cytoskeletal protein complex is essential for division of intracellular amastigotes of Leishmania mexicana

Felice D. Kelly, Khoa D. Tran, Jess Hatfield, Kat Schmidt, Marco A. Sanchez, Scott M. Landfear

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Previous studies in Leishmania mexicana have identified the cytoskeletal protein KHARON as being important for both flagellar trafficking of the glucose transporter GT1 and for successful cytokinesis and survival of infectious amastigote forms inside mammalian macrophages. KHARON is located in three distinct regions of the cytoskeleton: the base of the flagellum, the subpellicular microtubules, and the mitotic spindle. To deconvolve the different functions for KHARON, we have identified two partner proteins, KHAP1 and KHAP2, which associate with KHARON. KHAP1 is located only in the subpellicular microtubules, whereas KHAP2 is located at the subpellicular microtubules and the base of the flagellum. Both KHAP1 and KHAP2 null mutants are unable to execute cytokinesis but are able to traffic GT1 to the flagellum. These results confirm that KHARON assembles into distinct functional complexes and that the subpellicular complex is essential for cytokinesis and viability of disease-causing amastigotes but not for flagellar membrane trafficking.

Original languageEnglish (US)
Pages (from-to)13106-13122
Number of pages17
JournalJournal of Biological Chemistry
Volume295
Issue number37
DOIs
StatePublished - Sep 11 2020

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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