A cytolysin encoded by Salmonella is required for survival within macrophages

Stephen J. Libby, Werner Goebel, Albrecht Ludwig, Nancy Buchmeier, Frances Bowe, Ferric C. Fang, Donald G. Guiney, J. Glenn Songer, Fred Heffron

    Research output: Contribution to journalArticle

    158 Scopus citations

    Abstract

    A Salmonella gene encoding a cytolysin has been identified by screening for hemolysis on blood agar. DNA sequence analyses together with genetic mapping in Salmonella suggest that it is unrelated to other toxins or hemolysins. The gene (slyA) is present in every strain of Salmonella examined, in Shigella, and in enteroinvasive Escherichia coli but not in other Enterobacteriaceae. SlyA (salmolysin) purified from a derivative of the original clone has hemolytic and cytolytic activity and has a molecular weight predicted by the DNA sequence. The median lethal dose and infection kinetics in mice suggest that the toxin is required for virulence and facilitates Salmonella survival within mouse peritoneal macrophages.

    Original languageEnglish (US)
    Pages (from-to)489-493
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume91
    Issue number2
    DOIs
    StatePublished - Jan 18 1994

    Keywords

    • pathogenesis
    • toxin

    ASJC Scopus subject areas

    • General

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  • Cite this

    Libby, S. J., Goebel, W., Ludwig, A., Buchmeier, N., Bowe, F., Fang, F. C., Guiney, D. G., Songer, J. G., & Heffron, F. (1994). A cytolysin encoded by Salmonella is required for survival within macrophages. Proceedings of the National Academy of Sciences of the United States of America, 91(2), 489-493. https://doi.org/10.1073/pnas.91.2.489