A cytolysin encoded by Salmonella is required for survival within macrophages

Stephen J. Libby; Werner Goebel; Albrecht Ludwig; Nancy Buchmeier; Frances Bowe; Ferric C. Fang; Donald G. Guiney; J. Glenn Songer; Fred Heffron

doi:10.1073/pnas.91.2.489

A cytolysin encoded by Salmonella is required for survival within macrophages

Stephen J. Libby, Werner Goebel, Albrecht Ludwig, Nancy Buchmeier, Frances Bowe, Ferric C. Fang, Donald G. Guiney, J. Glenn Songer, Fred Heffron

Research output: Contribution to journal › Article › peer-review

165 Scopus citations

Abstract

A Salmonella gene encoding a cytolysin has been identified by screening for hemolysis on blood agar. DNA sequence analyses together with genetic mapping in Salmonella suggest that it is unrelated to other toxins or hemolysins. The gene (slyA) is present in every strain of Salmonella examined, in Shigella, and in enteroinvasive Escherichia coli but not in other Enterobacteriaceae. SlyA (salmolysin) purified from a derivative of the original clone has hemolytic and cytolytic activity and has a molecular weight predicted by the DNA sequence. The median lethal dose and infection kinetics in mice suggest that the toxin is required for virulence and facilitates Salmonella survival within mouse peritoneal macrophages.

Original language	English (US)
Pages (from-to)	489-493
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	2
DOIs	https://doi.org/10.1073/pnas.91.2.489
State	Published - Jan 18 1994
Externally published	Yes

Keywords

pathogenesis
toxin

ASJC Scopus subject areas

General

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title = "A cytolysin encoded by Salmonella is required for survival within macrophages",

abstract = "A Salmonella gene encoding a cytolysin has been identified by screening for hemolysis on blood agar. DNA sequence analyses together with genetic mapping in Salmonella suggest that it is unrelated to other toxins or hemolysins. The gene (slyA) is present in every strain of Salmonella examined, in Shigella, and in enteroinvasive Escherichia coli but not in other Enterobacteriaceae. SlyA (salmolysin) purified from a derivative of the original clone has hemolytic and cytolytic activity and has a molecular weight predicted by the DNA sequence. The median lethal dose and infection kinetics in mice suggest that the toxin is required for virulence and facilitates Salmonella survival within mouse peritoneal macrophages.",

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AU - Libby, Stephen J.

AU - Goebel, Werner

AU - Ludwig, Albrecht

AU - Buchmeier, Nancy

AU - Bowe, Frances

AU - Fang, Ferric C.

AU - Guiney, Donald G.

AU - Songer, J. Glenn

AU - Heffron, Fred

PY - 1994/1/18

Y1 - 1994/1/18

N2 - A Salmonella gene encoding a cytolysin has been identified by screening for hemolysis on blood agar. DNA sequence analyses together with genetic mapping in Salmonella suggest that it is unrelated to other toxins or hemolysins. The gene (slyA) is present in every strain of Salmonella examined, in Shigella, and in enteroinvasive Escherichia coli but not in other Enterobacteriaceae. SlyA (salmolysin) purified from a derivative of the original clone has hemolytic and cytolytic activity and has a molecular weight predicted by the DNA sequence. The median lethal dose and infection kinetics in mice suggest that the toxin is required for virulence and facilitates Salmonella survival within mouse peritoneal macrophages.

AB - A Salmonella gene encoding a cytolysin has been identified by screening for hemolysis on blood agar. DNA sequence analyses together with genetic mapping in Salmonella suggest that it is unrelated to other toxins or hemolysins. The gene (slyA) is present in every strain of Salmonella examined, in Shigella, and in enteroinvasive Escherichia coli but not in other Enterobacteriaceae. SlyA (salmolysin) purified from a derivative of the original clone has hemolytic and cytolytic activity and has a molecular weight predicted by the DNA sequence. The median lethal dose and infection kinetics in mice suggest that the toxin is required for virulence and facilitates Salmonella survival within mouse peritoneal macrophages.

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A cytolysin encoded by Salmonella is required for survival within macrophages

Abstract

Keywords

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