A cyclooxygenase-2 homologue encoded by rhesus cytomegalovirus is a determinant for endothelial cell tropism

Cary A. Rue, Michael A. Jarvis, Amber J. Knoche, Heather L. Meyers, Victor De Filippis, Scott Hansen, Markus Wagner, Klaus Frueh, David G. Anders, Scott Wong, Peter A. Barry, Jay Nelson

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Cyclooxygenase-2 (COX-2) is a cellular enzyme in the eicosanoid synthetic pathway that mediates the synthesis of prostaglandins from arachidonic acid. The eicosanoids function as critical regulators of a number of cellular processes, including the acute and chronic inflammatory response, hemostasis, and the innate immune response. Human cytomegalovirus (HCMV), which does not encode a viral COX-2 isoform, has been shown to induce cellular COX-2 expression. Importantly, although the precise role of COX-2 in CMV replication is unknown, COX-2 induction was shown to be critical for normal HCMV replication. In an earlier study, we identified an open reading frame (Rh10) within the rhesus cytomegalovirus (RhCMV) genome that encoded a putative protein (designated vCOX-2) with high homology to cellular COX-2. In the current study, we show that vCOX-2 is expressed with early-gene kinetics during RhCMV infection, resulting in production of a 70-kDa protein. Consistent with the expression of a viral COX-2 isoform, cellular COX-2 expression was not induced during RhCMV infection. Finally, analysis of growth of recombinant RhCMV with vCOX-2 deleted identified vCOX-2 as a critical determinant for replication in endothelial cells.

Original languageEnglish (US)
Pages (from-to)12529-12536
Number of pages8
JournalJournal of Virology
Volume78
Issue number22
DOIs
StatePublished - Nov 2004

Fingerprint

Cytomegalovirus
tropisms
Tropism
prostaglandin synthase
Cyclooxygenase 2
endothelial cells
Endothelial Cells
Human herpesvirus 5
eicosanoids
Eicosanoids
Cytomegalovirus Infections
Protein Isoforms
hemostasis
Hemostasis
arachidonic acid
Innate Immunity
Arachidonic Acid
prostaglandins
Open Reading Frames
Prostaglandins

ASJC Scopus subject areas

  • Immunology

Cite this

A cyclooxygenase-2 homologue encoded by rhesus cytomegalovirus is a determinant for endothelial cell tropism. / Rue, Cary A.; Jarvis, Michael A.; Knoche, Amber J.; Meyers, Heather L.; De Filippis, Victor; Hansen, Scott; Wagner, Markus; Frueh, Klaus; Anders, David G.; Wong, Scott; Barry, Peter A.; Nelson, Jay.

In: Journal of Virology, Vol. 78, No. 22, 11.2004, p. 12529-12536.

Research output: Contribution to journalArticle

Rue, Cary A. ; Jarvis, Michael A. ; Knoche, Amber J. ; Meyers, Heather L. ; De Filippis, Victor ; Hansen, Scott ; Wagner, Markus ; Frueh, Klaus ; Anders, David G. ; Wong, Scott ; Barry, Peter A. ; Nelson, Jay. / A cyclooxygenase-2 homologue encoded by rhesus cytomegalovirus is a determinant for endothelial cell tropism. In: Journal of Virology. 2004 ; Vol. 78, No. 22. pp. 12529-12536.
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