A Cyclic AMP-Responsive Element-Binding Transcriptional Activator in Drosophila melanogaster, dCREB-A, Is a Member of the Leucine Zipper Family

Sarah M. Smolik, Ronald E. Rose, Richard Goodman

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

In this report, we describe the isolation and initial characterization of a Drosophila protein, dCREB-A, that can bind the somatostatin cyclic AMP (cAMP)-responsive element and is capable of activating transcription in cell culture. Sequence analysis demonstrates that this protein is a member of the leucine zipper family of transcription factors. dCREB-A is unusual in that it contains six hydrophobic residue iterations in the zipper domain rather than the four or five commonly found in this group of proteins. The DNA-binding domain is more closely related to mammalian CREB than to the AP-1 factors in both sequence homology and specificity of cAMP-responsive element binding. In embryos, dCREB-A is expressed in the developing salivary gland. A more complex pattern of expression is detected in the adult; transcripts are found in the brain and optic lobe cell bodies, salivary gland, and midgut epithelial cells of the cardia. In females, dCREB-A is expressed in the ovarian columnar follicle cells, and in males, dCREB-A RNA is seen in the seminal vesicle, ejaculatory duct, and ejaculatory bulb. These results suggest that the dCREB-A transcription factor may be involved in fertility and neurological functions.

Original languageEnglish (US)
Pages (from-to)4123-4131
Number of pages9
JournalMolecular and Cellular Biology
Volume12
Issue number9
StatePublished - Sep 1992

Fingerprint

Leucine Zippers
Salivary Glands
Drosophila melanogaster
Cyclic AMP
Transcription Factors
Ejaculatory Ducts
Cardia
Ovarian Follicle
Seminal Vesicles
Protein Sequence Analysis
Transcription Factor AP-1
Sequence Homology
Somatostatin
Fertility
Embryonic Structures
Cell Culture Techniques
Epithelial Cells
RNA
DNA
Brain

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

A Cyclic AMP-Responsive Element-Binding Transcriptional Activator in Drosophila melanogaster, dCREB-A, Is a Member of the Leucine Zipper Family. / Smolik, Sarah M.; Rose, Ronald E.; Goodman, Richard.

In: Molecular and Cellular Biology, Vol. 12, No. 9, 09.1992, p. 4123-4131.

Research output: Contribution to journalArticle

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