TY - JOUR
T1 - A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state
AU - Suh, Se Won
AU - Cascio, Duilio
AU - Chapman, Michael S.
AU - Eisenberg, David
N1 - Funding Information:
We thank Dr ,J. Pierce, DuPont, Wilmington, for a generous gift of CABP and Dr P. Boon, Molecular Biology Institute, IJCLA, for carrying out the differential sedimentation experiment. This research has been supported by NIH grant GM31299 and the University of California Hioterhnology Research and Training Program.
PY - 1987/9/20
Y1 - 1987/9/20
N2 - A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC4.1.1.39) from Nicotiana tabacum has been obtained at alkaline pH with polyethylene glycol 8000 in the presence of a non-ionic detergent, β-octyl glucoside. The crystals are grown at room temperature by the hanging-drop vapor diffusion technique from a protein solution containing enzyme complexed with CO2, Mg2+, and the transition state analog 2-C-carboxy-d-arabinitol-1,5-bisphosphate. The crystals belong to the the space group P3121 (or P3221) with the cell parameters a = 204.6 A ̊, and c= 117.4 A ̊ (1 A ̊ = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 Å Bragg spacing and are suitable for X-ray structure determination.
AB - A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC4.1.1.39) from Nicotiana tabacum has been obtained at alkaline pH with polyethylene glycol 8000 in the presence of a non-ionic detergent, β-octyl glucoside. The crystals are grown at room temperature by the hanging-drop vapor diffusion technique from a protein solution containing enzyme complexed with CO2, Mg2+, and the transition state analog 2-C-carboxy-d-arabinitol-1,5-bisphosphate. The crystals belong to the the space group P3121 (or P3221) with the cell parameters a = 204.6 A ̊, and c= 117.4 A ̊ (1 A ̊ = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 Å Bragg spacing and are suitable for X-ray structure determination.
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U2 - 10.1016/0022-2836(87)90129-X
DO - 10.1016/0022-2836(87)90129-X
M3 - Article
C2 - 3681999
AN - SCOPUS:0023660966
SN - 0022-2836
VL - 197
SP - 363
EP - 365
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 2
ER -