A crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum in the activated state

Se Won Suh, Duilio Cascio, Michael S. Chapman, David Eisenberg

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A new crystal form of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC4.1.1.39) from Nicotiana tabacum has been obtained at alkaline pH with polyethylene glycol 8000 in the presence of a non-ionic detergent, β-octyl glucoside. The crystals are grown at room temperature by the hanging-drop vapor diffusion technique from a protein solution containing enzyme complexed with CO2, Mg2+, and the transition state analog 2-C-carboxy-d-arabinitol-1,5-bisphosphate. The crystals belong to the the space group P3121 (or P3221) with the cell parameters a = 204.6 A ̊, and c= 117.4 A ̊ (1 A ̊ = 0.1 nm). The asymmetric unit contains half (L4S4: L, large subunit, 53,000 Mr; S, small subunit, 15,000 Mr) of a hexadecameric molecule (L8S8, 540,000 Mr). The crystals diffract to at least 2.6 Å Bragg spacing and are suitable for X-ray structure determination.

Original languageEnglish (US)
Pages (from-to)363-365
Number of pages3
JournalJournal of molecular biology
Volume197
Issue number2
DOIs
StatePublished - Sep 20 1987

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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