@article{46810b9820804a0397d6ecfd2af3e3b9,
title = "A comprehensive thalamocortical projection map at the mesoscopic level",
abstract = "The thalamus relays sensori-motor information to the cortex and is an integral part of cortical executive functions. The precise distribution of thalamic projections to the cortex is poorly characterized, particularly in mouse. We employed a systematic, high-throughput viral approach to visualize thalamocortical axons with high sensitivity. We then developed algorithms to directly compare injection and projection information across animals. By tiling the mouse thalamus with 254 overlapping injections, we constructed a comprehensive map of thalamocortical projections. We determined the projection origins of specific cortical subregions and verified that the characterized projections formed functional synapses using optogenetic approaches. As an important application, we determined the optimal stereotaxic coordinates for targeting specific cortical subregions and expanded these analyses to localize cortical layer-preferential projections. This data set will serve as a foundation for functional investigations of thalamocortical circuits. Our approach and algorithms also provide an example for analyzing the projection patterns of other brain regions.",
author = "Hunnicutt, {Barbara J.} and Long, {Brian R.} and Deniz Kusefoglu and Gertz, {Katrina J.} and Haining Zhong and Tianyi Mao",
note = "Funding Information: All Nanozoomer images were collected in Janelia Farm Research Campus. We thank C. Mello, M. Qin, J. Qiu, J. Li, S.K. Petrie, A. Gilmore, Y. Zuo and D. Lioy for technical support. We thank K. Svoboda, J. Lichtman, K. Huang and B. Li for their discussions, and K. Svoboda, J. Williams and J. Adelman for comments on the manuscript. We thank R. Champieux and K. Banerjee for establishing the web data depository. We thank K. Svoboda and HHMI, Janelia Farm Research Campus for their generous support in the initiation and data collection phase of this project. This work was supported by an American Recovery and Reinvestment Act grant (P30 NS069305, R. Goodman), a National Science Foundation Graduate Research Fellowship Program fellowship (B.J.H.), Achievement Rewards for College Scientists Foundation Portland Chapter (B.J.H.), a US National Institutes of Health Director{\textquoteright}s Innovator Award (DP2 OD008425, H.Z.), a US National Institutes of Health R01 grant (R01 NS081071, T.M.) and the Medical Research Foundation (T.M.). Publisher Copyright: {\textcopyright} 2014 Nature America, Inc. All rights reserved.",
year = "2014",
month = sep,
day = "1",
doi = "10.1038/nn.3780",
language = "English (US)",
volume = "17",
pages = "1276--1285",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "Nature Publishing Group",
number = "9",
}