Abstract
The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gene trap experiments were tested for their ability to colonize the germline. The maximal g.l.t. age for wild-type ES cells was equal to passage 26 and for transfected clones was equivalent to passage 32 of parental links. It is shown that wild-type ES cells of less than a passage 15 should be used for effective production of transgenic g.l.t. clones. A simple system is outlined to evaluate the probability of g.l.t. on the basis of the chimaeric progeny obtained.
Original language | English (US) |
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Pages (from-to) | 223-231 |
Number of pages | 9 |
Journal | Transgenic Research |
Volume | 6 |
Issue number | 3 |
DOIs | |
State | Published - 1997 |
Externally published | Yes |
Keywords
- ES-embryonic stem cells
- chimaera
- g.l.t.-germline transmission
- mouse
- passage
- transgenic
ASJC Scopus subject areas
- Biotechnology
- Animal Science and Zoology
- Agronomy and Crop Science
- Genetics