A comparison of the germline potential of differently aged ES cell lines and their transfected descendants

Lev Fedorov, Hélène Haegel-Kronenberger, Jens Hirchenhain

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gene trap experiments were tested for their ability to colonize the germline. The maximal g.l.t. age for wild-type ES cells was equal to passage 26 and for transfected clones was equivalent to passage 32 of parental links. It is shown that wild-type ES cells of less than a passage 15 should be used for effective production of transgenic g.l.t. clones. A simple system is outlined to evaluate the probability of g.l.t. on the basis of the chimaeric progeny obtained.

Original languageEnglish (US)
Pages (from-to)223-231
Number of pages9
JournalTransgenic Research
Volume6
Issue number3
StatePublished - May 1997
Externally publishedYes

Fingerprint

germ cells
Clone Cells
cell lines
Cell Line
Genes
clones
Gene Targeting
traps
genetically modified organisms
genes
gene targeting
cells
Mutation
mutation
mice

Keywords

  • chimaera
  • ES-embryonic stem cells
  • g.l.t.-germline transmission
  • mouse
  • passage
  • transgenic

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Biotechnology
  • Food Science

Cite this

A comparison of the germline potential of differently aged ES cell lines and their transfected descendants. / Fedorov, Lev; Haegel-Kronenberger, Hélène; Hirchenhain, Jens.

In: Transgenic Research, Vol. 6, No. 3, 05.1997, p. 223-231.

Research output: Contribution to journalArticle

Fedorov, Lev ; Haegel-Kronenberger, Hélène ; Hirchenhain, Jens. / A comparison of the germline potential of differently aged ES cell lines and their transfected descendants. In: Transgenic Research. 1997 ; Vol. 6, No. 3. pp. 223-231.
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