A comparison of the germline potential of differently aged ES cell lines and their transfected descendants

Lev M. Fedorov, Hélène Haegel-Kronenberger, Jens Hirchenhain

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gene trap experiments were tested for their ability to colonize the germline. The maximal g.l.t. age for wild-type ES cells was equal to passage 26 and for transfected clones was equivalent to passage 32 of parental links. It is shown that wild-type ES cells of less than a passage 15 should be used for effective production of transgenic g.l.t. clones. A simple system is outlined to evaluate the probability of g.l.t. on the basis of the chimaeric progeny obtained.

Original languageEnglish (US)
Pages (from-to)223-231
Number of pages9
JournalTransgenic Research
Volume6
Issue number3
DOIs
StatePublished - 1997
Externally publishedYes

Keywords

  • ES-embryonic stem cells
  • chimaera
  • g.l.t.-germline transmission
  • mouse
  • passage
  • transgenic

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Agronomy and Crop Science
  • Genetics

Fingerprint Dive into the research topics of 'A comparison of the germline potential of differently aged ES cell lines and their transfected descendants'. Together they form a unique fingerprint.

Cite this