A comparison of the germline potential of differently aged ES cell lines and their transfected descendants

Lev M. Fedorov, Hélène Haegel-Kronenberger, Jens Hirchenhain

    Research output: Contribution to journalArticlepeer-review

    20 Scopus citations

    Abstract

    The germline transmission (g.l.t.) of gene trap or gene targeted mutations by ES-cell-derived chimaeric mice is a crucial step in the generation of stable transgenic lines. The wild-type ES cell lines CJ7, D3 and R1 of different passage numbers and their transfected clone-descendants generated in gene targeting or gene trap experiments were tested for their ability to colonize the germline. The maximal g.l.t. age for wild-type ES cells was equal to passage 26 and for transfected clones was equivalent to passage 32 of parental links. It is shown that wild-type ES cells of less than a passage 15 should be used for effective production of transgenic g.l.t. clones. A simple system is outlined to evaluate the probability of g.l.t. on the basis of the chimaeric progeny obtained.

    Original languageEnglish (US)
    Pages (from-to)223-231
    Number of pages9
    JournalTransgenic Research
    Volume6
    Issue number3
    DOIs
    StatePublished - Jan 1 1997

    Keywords

    • ES-embryonic stem cells
    • chimaera
    • g.l.t.-germline transmission
    • mouse
    • passage
    • transgenic

    ASJC Scopus subject areas

    • Biotechnology
    • Animal Science and Zoology
    • Agronomy and Crop Science
    • Genetics

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