A comparison of the crystallographic structures of two catalytic antibodies with esterase activity

Jenny L. Buchbinder, Robert C. Stephenson, Thomas (Tom) Scanlan, Robert J. Fletterick

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The crystallographic structure of the Fab fragment of the catalytic antibody, 29G11, complexed with an (S)-norleucine phenyl phosphonate transition state analog was determined at 2.2 Å resolution. The antibody catalyzes the hydrolysis of norleucine phenyl ester with (S)-enantioselectivity. The shape and charge complementarity of the binding pocket for the hapten account for the preferential binding of the (S)-enantiomer of the substrate. The structure is compared to that of the more catalytically efficient antibody, 17E8, induced by the same hapten transition state analog. 29G11 has different residues from 17E8 at eight positions in the heavy chain, including four substitutions in the hapten-binding pocket: A33V, S95G, S99R and Y100(A)N, and four substitutions at positions remote from the catalytic site, I28T, R40K, V65G and F91L. The two antibodies show large differences in the orientations of their variable and constant domains, reflected by a 32°difference in their elbow angles. The V(L) and V(H) domains in the two antibodies differ by a rotation of 8.8°. The hapten binds in similar orientations and locations in 29G11 and 17E8, which appear to have catalytic groups in common, though the changes in the association of the variable domains affect the precise positioning of residues in the hapten-binding pocket.

Original languageEnglish (US)
Pages (from-to)1033-1041
Number of pages9
JournalJournal of Molecular Biology
Volume282
Issue number5
DOIs
StatePublished - Oct 9 1998
Externally publishedYes

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Catalytic Antibodies
Haptens
Esterases
Norleucine
Antibodies
Organophosphonates
Immunoglobulin Fab Fragments
Elbow
Catalytic Domain
Esters
Hydrolysis

Keywords

  • Catalytic antibody
  • Esterase
  • Structure
  • Transition state analog
  • X-ray crystallography

ASJC Scopus subject areas

  • Virology

Cite this

A comparison of the crystallographic structures of two catalytic antibodies with esterase activity. / Buchbinder, Jenny L.; Stephenson, Robert C.; Scanlan, Thomas (Tom); Fletterick, Robert J.

In: Journal of Molecular Biology, Vol. 282, No. 5, 09.10.1998, p. 1033-1041.

Research output: Contribution to journalArticle

Buchbinder, Jenny L. ; Stephenson, Robert C. ; Scanlan, Thomas (Tom) ; Fletterick, Robert J. / A comparison of the crystallographic structures of two catalytic antibodies with esterase activity. In: Journal of Molecular Biology. 1998 ; Vol. 282, No. 5. pp. 1033-1041.
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