A comparison of the cell lines used in meningioma research

Brian T. Ragel, William T. Couldwell, David L. Gillespie, Merideth M. Wendland, Kum Whang, Randy L. Jensen

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: Immortal cell lines and cell lines derived from operative specimens transplanted into animal models are used in meningioma research. We address 2 criticisms of the mouse xenograft flank tumor model: Why are tumor induction rates derived from operative specimens low and inconsistent? Are flank tumors meningiomas? Methods: Meningioma cell cultures were processed for Giemsa-band karyotyping and flow cytometry. Mouse flank tumors induced subcutaneously were analyzed microscopically, immunohistochemically, and ultrastructurally. Giemsa-band studies identified meningiomas with simple karyotype (≤1 chromosomal abnormality) or complex karyotype (multiple chromosomal abnormalities). Results: Cell cultures with complex karyotypes (IOMM-Lee, CH-157 MN, 2 operative specimens) grew rapidly in vitro and induced tumors in 49 (98%) of 50 animals. Meningioma cell cultures with simple karyotypes grew slowly in vitro and showed small, nongrowing tumors in mouse flanks (10/10). Meningioma flank tumors were vimentin-positive with ultrastructural features consistent with meningiomas. Cell cultures with complex karyotypes grew faster in cell culture and consistently induced flank tumors, unlike meningiomas with simple karyotypes. Conclusions: Meningioma cell lines transplanted into flanks of nude mice exhibit microscopic, immunohistochemical, and ultrastructural features of meningiomas. The ease of monitoring tumor growth in the subcutaneous mouse flank model is its primary advantage, although we recognize an intracranial location is more biologically desirable.

Original languageEnglish (US)
Pages (from-to)295-307
Number of pages13
JournalSurgical Neurology
Volume70
Issue number3
DOIs
StatePublished - Sep 2008
Externally publishedYes

Fingerprint

Meningioma
Cell Line
Karyotype
Research
Cell Culture Techniques
Neoplasms
Chromosome Aberrations
Multiple Abnormalities
Karyotyping
Vimentin
Heterografts
Nude Mice
Flow Cytometry
Animal Models

Keywords

  • CH-157 MN
  • IOMM-Lee
  • Meningioma
  • Xenograft mouse model

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Ragel, B. T., Couldwell, W. T., Gillespie, D. L., Wendland, M. M., Whang, K., & Jensen, R. L. (2008). A comparison of the cell lines used in meningioma research. Surgical Neurology, 70(3), 295-307. https://doi.org/10.1016/j.surneu.2007.06.031

A comparison of the cell lines used in meningioma research. / Ragel, Brian T.; Couldwell, William T.; Gillespie, David L.; Wendland, Merideth M.; Whang, Kum; Jensen, Randy L.

In: Surgical Neurology, Vol. 70, No. 3, 09.2008, p. 295-307.

Research output: Contribution to journalArticle

Ragel, BT, Couldwell, WT, Gillespie, DL, Wendland, MM, Whang, K & Jensen, RL 2008, 'A comparison of the cell lines used in meningioma research', Surgical Neurology, vol. 70, no. 3, pp. 295-307. https://doi.org/10.1016/j.surneu.2007.06.031
Ragel BT, Couldwell WT, Gillespie DL, Wendland MM, Whang K, Jensen RL. A comparison of the cell lines used in meningioma research. Surgical Neurology. 2008 Sep;70(3):295-307. https://doi.org/10.1016/j.surneu.2007.06.031
Ragel, Brian T. ; Couldwell, William T. ; Gillespie, David L. ; Wendland, Merideth M. ; Whang, Kum ; Jensen, Randy L. / A comparison of the cell lines used in meningioma research. In: Surgical Neurology. 2008 ; Vol. 70, No. 3. pp. 295-307.
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