### Abstract

Twelve methods for analysing FCM-histograms were compared using the same set of data. Some of the histograms that were analysed were simulated by computer and some were taken from experiments. Simulated data were generated assuming asynchronously growing cell populations and (i) measurement coefficients of variations (CV) from 2 to 16%; (ii) constant measurements CV or CV's increasing from G_{1} to G_{2} phase, and (iii) varying fractions of cells in each phase. Simulated data were also generated assuming synchronous cell populations in which a block in early S phase was applied and released. DNA histograms were measured for L-929 cells at various times after mitotic selection. Labelling indices were also measured for these cells at the same time. The fractions of cells in the G_{1}, S, and (G_{2} + M) phases were calculated by each analytical method and compared with the actual fractions used for simulation, or in case of experimental data, with autoradiographic results. Generally, all methods yielded reasonably accurate fractions of cells in each phase with relative errors in the range of 10-20%. However, most methods tended to overestimate G_{1} fractions and underestimate S fractions. In addition, variations in the shape of the S phase distribution often caused considerable errors. Phase fractions were also calculated for histograms of kinetically perturbed populations, simulated as well as experimental. The errors were only slightly larger than for histograms from asynchronously growing cell populations.

Original language | English (US) |
---|---|

Pages (from-to) | 235-249 |

Number of pages | 15 |

Journal | Cell and Tissue Kinetics |

Volume | 15 |

Issue number | 3 |

State | Published - 1982 |

Externally published | Yes |

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### ASJC Scopus subject areas

- Cell Biology

### Cite this

*Cell and Tissue Kinetics*,

*15*(3), 235-249.

**A comparison of mathematical methods for the analysis of DNA histograms obtained by flow cytometry.** / Baisch, H.; Beck, H. P.; Christensen, I. J.; Hartmann, N. R.; Fried, J.; Dean, P. N.; Gray, Joe; Jett, J. H.; Johnston, D. A.; White, R. A.; Nicolini, C.; Zeitz, S.; Watson, J. V.

Research output: Contribution to journal › Article

*Cell and Tissue Kinetics*, vol. 15, no. 3, pp. 235-249.

}

TY - JOUR

T1 - A comparison of mathematical methods for the analysis of DNA histograms obtained by flow cytometry

AU - Baisch, H.

AU - Beck, H. P.

AU - Christensen, I. J.

AU - Hartmann, N. R.

AU - Fried, J.

AU - Dean, P. N.

AU - Gray, Joe

AU - Jett, J. H.

AU - Johnston, D. A.

AU - White, R. A.

AU - Nicolini, C.

AU - Zeitz, S.

AU - Watson, J. V.

PY - 1982

Y1 - 1982

N2 - Twelve methods for analysing FCM-histograms were compared using the same set of data. Some of the histograms that were analysed were simulated by computer and some were taken from experiments. Simulated data were generated assuming asynchronously growing cell populations and (i) measurement coefficients of variations (CV) from 2 to 16%; (ii) constant measurements CV or CV's increasing from G1 to G2 phase, and (iii) varying fractions of cells in each phase. Simulated data were also generated assuming synchronous cell populations in which a block in early S phase was applied and released. DNA histograms were measured for L-929 cells at various times after mitotic selection. Labelling indices were also measured for these cells at the same time. The fractions of cells in the G1, S, and (G2 + M) phases were calculated by each analytical method and compared with the actual fractions used for simulation, or in case of experimental data, with autoradiographic results. Generally, all methods yielded reasonably accurate fractions of cells in each phase with relative errors in the range of 10-20%. However, most methods tended to overestimate G1 fractions and underestimate S fractions. In addition, variations in the shape of the S phase distribution often caused considerable errors. Phase fractions were also calculated for histograms of kinetically perturbed populations, simulated as well as experimental. The errors were only slightly larger than for histograms from asynchronously growing cell populations.

AB - Twelve methods for analysing FCM-histograms were compared using the same set of data. Some of the histograms that were analysed were simulated by computer and some were taken from experiments. Simulated data were generated assuming asynchronously growing cell populations and (i) measurement coefficients of variations (CV) from 2 to 16%; (ii) constant measurements CV or CV's increasing from G1 to G2 phase, and (iii) varying fractions of cells in each phase. Simulated data were also generated assuming synchronous cell populations in which a block in early S phase was applied and released. DNA histograms were measured for L-929 cells at various times after mitotic selection. Labelling indices were also measured for these cells at the same time. The fractions of cells in the G1, S, and (G2 + M) phases were calculated by each analytical method and compared with the actual fractions used for simulation, or in case of experimental data, with autoradiographic results. Generally, all methods yielded reasonably accurate fractions of cells in each phase with relative errors in the range of 10-20%. However, most methods tended to overestimate G1 fractions and underestimate S fractions. In addition, variations in the shape of the S phase distribution often caused considerable errors. Phase fractions were also calculated for histograms of kinetically perturbed populations, simulated as well as experimental. The errors were only slightly larger than for histograms from asynchronously growing cell populations.

UR - http://www.scopus.com/inward/record.url?scp=0020052746&partnerID=8YFLogxK

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M3 - Article

VL - 15

SP - 235

EP - 249

JO - Cell Proliferation

JF - Cell Proliferation

SN - 0960-7722

IS - 3

ER -