A cloning assay for 6-thioguanine resistance provides evidence against certain somatic mutational theories of aging

P. L. Horn, Mitchell Turker, C. E. Ogburn, C. M. Disteche, G. M. Martin

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The frequencies of 6-thioguanine-resistant primary clones from the kidneys and skeletal muscles of aging male cohorts of two F1 hybrid strains of Mus musculus varied from 0.59 to 10.96 x 10-5 and did not increase as a function of donor age (up to 40 months). Resistant clones were shown to be severely deficient in the activity of hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8). These deficiencies presumably resulted from molecular alterations at this X-linked locus, including point mutations. No alterations of the X-chromosome were observed at the level of the light microscope. These results are inconsistent with predictions of the intrinsic mutagenesis and protein synthesis error catastrophe theories of aging. They do not rule out, however, somatic mutational theories that evoke comparatively large-scale chromosomal lesions, many of which would be likely to be lethal at the cellular level.

Original languageEnglish (US)
Pages (from-to)309-315
Number of pages7
JournalJournal of Cellular Physiology
Volume121
Issue number2
StatePublished - 1984
Externally publishedYes

Fingerprint

Thioguanine
Cloning
Organism Cloning
Assays
Clone Cells
Aging of materials
Hypoxanthine Phosphoribosyltransferase
Mutagenesis
X Chromosome
Chromosomes
Point Mutation
Muscle
Skeletal Muscle
Microscopes
Kidney
Light
Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

A cloning assay for 6-thioguanine resistance provides evidence against certain somatic mutational theories of aging. / Horn, P. L.; Turker, Mitchell; Ogburn, C. E.; Disteche, C. M.; Martin, G. M.

In: Journal of Cellular Physiology, Vol. 121, No. 2, 1984, p. 309-315.

Research output: Contribution to journalArticle

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