A cloned bovine cDNA encodes an alternate form of the catalytic subunit of cAMP-dependent protein kinase

M. O. Showers, Richard Maurer

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

While attempting to isolate a cDNA clone for the catalytic subunit of the bovine cAMP-dependent protein kinase, we have isolated cDNAs which code for a protein slightly different than the known amino acid sequence. The alternate cDNA was identified by screening a bovine pituitary cDNA library using synthetic oligonucleotides predicted from the known amino acid sequence of the catalytic subunit. The cDNA which we identified, encodes a protein which is 93% identical to the known amino acid sequence of the bovine catalytic subunit. It seems likely that this cDNA represents a previously undiscovered catalytic subunit of the cAMP-dependent protein kinase. The mRNA for the alternate catalytic subunit is different in size from the mRNA coding for the previously known catalytic subunit and also has a different tissue distribution. These findings suggest that there are at least two different genes for the catalytic subunit. The differences in amino acid sequence and tissue distribution suggest the possibility of important functional differences in the two enzymes.

Original languageEnglish (US)
Pages (from-to)16288-16291
Number of pages4
JournalJournal of Biological Chemistry
Volume261
Issue number35
StatePublished - 1986
Externally publishedYes

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Cyclic AMP-Dependent Protein Kinases
Catalytic Domain
Complementary DNA
Amino Acids
Amino Acid Sequence
Tissue Distribution
Tissue
Messenger RNA
Gene Library
Oligonucleotides
Screening
Proteins
Genes
Clone Cells
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

A cloned bovine cDNA encodes an alternate form of the catalytic subunit of cAMP-dependent protein kinase. / Showers, M. O.; Maurer, Richard.

In: Journal of Biological Chemistry, Vol. 261, No. 35, 1986, p. 16288-16291.

Research output: Contribution to journalArticle

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