A class II histone deacetylase acts on newly synthesized histones in tetrahymena

Joshua J. Smith, Sharon E. Torigoe, Julia Maxson, Lisa C. Fish, Emily A. Wiley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Newly synthesized histones are acetylated prior to their deposition into nucleosomes. Following nucleosome formation and positioning, they are rapidly deacetylated, an event that coincides with further maturation of the chromatin fiber. The histone deacetylases (HDACs) used for histone deposition and de novo chromatin formation are poorly understood. In the ciliate Tetrahymena thermophila, transcription-related deacetylation in the macronucleus is physically separated from deposition-related deacetylation in the micronucleus. This feature was utilized to identify an HDAC named Thd2, a class II HDAC that acts on newly synthesized histones to remove deposition-related acetyl moieties. The THD2 transcript is alternatively spliced, and the major form contains a putative inositol polyphosphate kinase (IPK) domain similar to Ipk2, an enzyme that promotes chromatin remodeling by SWI/SNF remodeling complexes. Cells lacking Thd2, which retain deposition-related acetyl moieties on new histones, exhibit chromatin and cytological phenotypes indicative of a role for Thd2 in chromatin maturation, including the proteolytic processing of histone H3.

Original languageEnglish (US)
Pages (from-to)471-482
Number of pages12
JournalEukaryotic Cell
Volume7
Issue number3
DOIs
StatePublished - Mar 1 2008

Fingerprint

Tetrahymena
Histone Deacetylases
Histones
Chromatin
Nucleosomes
Macronucleus
Tetrahymena thermophila
Chromatin Assembly and Disassembly
Inositol
Phenotype
Enzymes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

A class II histone deacetylase acts on newly synthesized histones in tetrahymena. / Smith, Joshua J.; Torigoe, Sharon E.; Maxson, Julia; Fish, Lisa C.; Wiley, Emily A.

In: Eukaryotic Cell, Vol. 7, No. 3, 01.03.2008, p. 471-482.

Research output: Contribution to journalArticle

Smith, Joshua J. ; Torigoe, Sharon E. ; Maxson, Julia ; Fish, Lisa C. ; Wiley, Emily A. / A class II histone deacetylase acts on newly synthesized histones in tetrahymena. In: Eukaryotic Cell. 2008 ; Vol. 7, No. 3. pp. 471-482.
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