A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells

Jeffrey R. Holt, Susan K H Gillespie, D. William Provance, Kavita Shah, Kevan M. Shokat, David P. Corey, John A. Mercer, Peter Barr-Gillespie

Research output: Contribution to journalArticle

238 Citations (Scopus)

Abstract

Myosin-1c (also known as myosin-Iβ) has been proposed to mediate the slow component of adaptation by hair cells, the sensory cells of the inner ear. To test this hypothesis, we mutated tyrosine-61 of myosin-1c to glycine, conferring susceptibility to inhibition by N6-modified ADP analogs. We expressed the mutant myosin-1c in utricular hair cells of transgenic mice, delivered an ADP analog through a whole-cell recording pipette, and found that the analog rapidly blocked adaptation to positive and negative deflections in transgenic cells but not in wild-type cells. The speed and specificity of inhibition suggests that myosin-1c participates in adaptation in hair cells.

Original languageEnglish (US)
Pages (from-to)371-381
Number of pages11
JournalCell
Volume108
Issue number3
DOIs
StatePublished - 2002

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Myosins
Cells
Adenosine Diphosphate
Myosin Type I
Glycine
Tyrosine
Patch-Clamp Techniques
Inner Ear
Transgenic Mice

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Holt, J. R., Gillespie, S. K. H., Provance, D. W., Shah, K., Shokat, K. M., Corey, D. P., ... Barr-Gillespie, P. (2002). A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells. Cell, 108(3), 371-381. https://doi.org/10.1016/S0092-8674(02)00629-3

A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells. / Holt, Jeffrey R.; Gillespie, Susan K H; Provance, D. William; Shah, Kavita; Shokat, Kevan M.; Corey, David P.; Mercer, John A.; Barr-Gillespie, Peter.

In: Cell, Vol. 108, No. 3, 2002, p. 371-381.

Research output: Contribution to journalArticle

Holt, JR, Gillespie, SKH, Provance, DW, Shah, K, Shokat, KM, Corey, DP, Mercer, JA & Barr-Gillespie, P 2002, 'A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells', Cell, vol. 108, no. 3, pp. 371-381. https://doi.org/10.1016/S0092-8674(02)00629-3
Holt JR, Gillespie SKH, Provance DW, Shah K, Shokat KM, Corey DP et al. A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells. Cell. 2002;108(3):371-381. https://doi.org/10.1016/S0092-8674(02)00629-3
Holt, Jeffrey R. ; Gillespie, Susan K H ; Provance, D. William ; Shah, Kavita ; Shokat, Kevan M. ; Corey, David P. ; Mercer, John A. ; Barr-Gillespie, Peter. / A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells. In: Cell. 2002 ; Vol. 108, No. 3. pp. 371-381.
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