@article{884f7b1c91c244d0858fd8d74cc9382d,
title = "A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells",
abstract = "Myosin-1c (also known as myosin-Iβ) has been proposed to mediate the slow component of adaptation by hair cells, the sensory cells of the inner ear. To test this hypothesis, we mutated tyrosine-61 of myosin-1c to glycine, conferring susceptibility to inhibition by N6-modified ADP analogs. We expressed the mutant myosin-1c in utricular hair cells of transgenic mice, delivered an ADP analog through a whole-cell recording pipette, and found that the analog rapidly blocked adaptation to positive and negative deflections in transgenic cells but not in wild-type cells. The speed and specificity of inhibition suggests that myosin-1c participates in adaptation in hair cells.",
author = "Holt, {Jeffrey R.} and Gillespie, {Susan K.H.} and Provance, {D. William} and Kavita Shah and Shokat, {Kevan M.} and Corey, {David P.} and Mercer, {John A.} and Gillespie, {Peter G.}",
note = "Funding Information: We thank Donna Klinedinst (Johns Hopkins University) for assembly of the original transgenic construct, Dee Goss (McLaughlin Research Institute) for oocyte injections, Colleen Silan (McLaughlin Research Institute) for genotyping, and Kevin Nusser (Oregon Health and Science University) for hair-bundle isolation and genotyping. This work was supported by NIH grants DC03279 (J.A.M., P.G.G., and J.R.H.), CA70331 (K.M.S.), DC00304 (D.P.C.), and DC02368 (P.G.G.). D.W.P. was supported by the Oberkotter Foundation and D.P.C. is an investigator with the Howard Hughes Medical Institute.",
year = "2002",
doi = "10.1016/S0092-8674(02)00629-3",
language = "English (US)",
volume = "108",
pages = "371--381",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",
}