A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells

Jeffrey R. Holt, Susan K.H. Gillespie, D. William Provance, Kavita Shah, Kevan M. Shokat, David P. Corey, John A. Mercer, Peter G. Gillespie

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246 Scopus citations

Abstract

Myosin-1c (also known as myosin-Iβ) has been proposed to mediate the slow component of adaptation by hair cells, the sensory cells of the inner ear. To test this hypothesis, we mutated tyrosine-61 of myosin-1c to glycine, conferring susceptibility to inhibition by N6-modified ADP analogs. We expressed the mutant myosin-1c in utricular hair cells of transgenic mice, delivered an ADP analog through a whole-cell recording pipette, and found that the analog rapidly blocked adaptation to positive and negative deflections in transgenic cells but not in wild-type cells. The speed and specificity of inhibition suggests that myosin-1c participates in adaptation in hair cells.

Original languageEnglish (US)
Pages (from-to)371-381
Number of pages11
JournalCell
Volume108
Issue number3
DOIs
StatePublished - Jan 1 2002

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Holt, J. R., Gillespie, S. K. H., Provance, D. W., Shah, K., Shokat, K. M., Corey, D. P., Mercer, J. A., & Gillespie, P. G. (2002). A chemical-genetic strategy implicates myosin-1c in adaptation by hair cells. Cell, 108(3), 371-381. https://doi.org/10.1016/S0092-8674(02)00629-3