A brain tumor molecular imaging strategy using a new triple-modality MRI-photoacoustic-Raman nanoparticle

Moritz F. Kircher, Adam De La Zerda, Jesse V. Jokerst, Cristina L. Zavaleta, Paul J. Kempen, Erik Mittra, Ken Pitter, Ruimin Huang, Carl Campos, Frezghi Habte, Robert Sinclair, Cameron W. Brennan, Ingo K. Mellinghoff, Eric C. Holland, Sanjiv S. Gambhir

Research output: Contribution to journalArticle

595 Citations (Scopus)

Abstract

The difficulty in delineating brain tumor margins is a major obstacle in the path toward better outcomes for patients with brain tumors. Current imaging methods are often limited by inadequate sensitivity, specificity and spatial resolution. Here we show that a unique triple-modality magnetic resonance imaging-photoacoustic imaging-Raman imaging nanoparticle (termed here MPR nanoparticle) can accurately help delineate the margins of brain tumors in living mice both preoperatively and intraoperatively. The MPRs were detected by all three modalities with at least a picomolar sensitivity both in vitro and in living mice. Intravenous injection of MPRs into glioblastoma-bearing mice led to MPR accumulation and retention by the tumors, with no MPR accumulation in the surrounding healthy tissue, allowing for a noninvasive tumor delineation using all three modalities through the intact skull. Raman imaging allowed for guidance of intraoperative tumor resection, and a histological correlation validated that Raman imaging was accurately delineating the brain tumor margins. This new triple-modality-nanoparticle approach has promise for enabling more accurate brain tumor imaging and resection.

Original languageEnglish (US)
Pages (from-to)829-834
Number of pages6
JournalNature medicine
Volume18
Issue number5
DOIs
StatePublished - May 1 2012
Externally publishedYes

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Molecular imaging
Molecular Imaging
Photoacoustic effect
Brain Neoplasms
Nanoparticles
Magnetic resonance imaging
Tumors
Brain
Imaging techniques
Neoplasms
Glioblastoma
Bearings (structural)
Skull
Neuroimaging
Intravenous Injections
Magnetic Resonance Imaging
Magnetic resonance
Sensitivity and Specificity
Tissue

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kircher, M. F., De La Zerda, A., Jokerst, J. V., Zavaleta, C. L., Kempen, P. J., Mittra, E., ... Gambhir, S. S. (2012). A brain tumor molecular imaging strategy using a new triple-modality MRI-photoacoustic-Raman nanoparticle. Nature medicine, 18(5), 829-834. https://doi.org/10.1038/nm.2721

A brain tumor molecular imaging strategy using a new triple-modality MRI-photoacoustic-Raman nanoparticle. / Kircher, Moritz F.; De La Zerda, Adam; Jokerst, Jesse V.; Zavaleta, Cristina L.; Kempen, Paul J.; Mittra, Erik; Pitter, Ken; Huang, Ruimin; Campos, Carl; Habte, Frezghi; Sinclair, Robert; Brennan, Cameron W.; Mellinghoff, Ingo K.; Holland, Eric C.; Gambhir, Sanjiv S.

In: Nature medicine, Vol. 18, No. 5, 01.05.2012, p. 829-834.

Research output: Contribution to journalArticle

Kircher, MF, De La Zerda, A, Jokerst, JV, Zavaleta, CL, Kempen, PJ, Mittra, E, Pitter, K, Huang, R, Campos, C, Habte, F, Sinclair, R, Brennan, CW, Mellinghoff, IK, Holland, EC & Gambhir, SS 2012, 'A brain tumor molecular imaging strategy using a new triple-modality MRI-photoacoustic-Raman nanoparticle', Nature medicine, vol. 18, no. 5, pp. 829-834. https://doi.org/10.1038/nm.2721
Kircher, Moritz F. ; De La Zerda, Adam ; Jokerst, Jesse V. ; Zavaleta, Cristina L. ; Kempen, Paul J. ; Mittra, Erik ; Pitter, Ken ; Huang, Ruimin ; Campos, Carl ; Habte, Frezghi ; Sinclair, Robert ; Brennan, Cameron W. ; Mellinghoff, Ingo K. ; Holland, Eric C. ; Gambhir, Sanjiv S. / A brain tumor molecular imaging strategy using a new triple-modality MRI-photoacoustic-Raman nanoparticle. In: Nature medicine. 2012 ; Vol. 18, No. 5. pp. 829-834.
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