A bolus of recombinant human follicle stimulating hormone at midcycle induces periovulatory events following multiple follicular development in macaques

M. B. Zelinski-Wooten, J. S. Hutchison, D. L. Hess, D. P. WoIf, R. L. Stouffer

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The efficacy of follicle stimulating hormone (FSH) as an alternative to luteinizing hormone (LH)/human chorionic gonadotrophin (HCG) for the initiation of periovulatory events in primate follicles is unknown. A single bolus of 2500 IU recombinant (r)-hFSH was compared to 1000 IU r-HCG for its ability to promote oocyte nuclear maturation and fertilization, granulosa cell luteinization and corpus luteum function following r-hFSH (60 IU/day) induction of multiple follicular development in rhesus monkeys. Following the r-hFSH bolus, bioactive luteinizing hormone concentrations were < 3 ng/ml. Peak concentrations of serum FSH (1455 ± 314 mIU/ml; mean ± SEM) were attained 2-8 h after r-hFSH, and declined by 96 h. Bioactive HCG concentrations peaked between 2-8 h after r-HCG and remained ≤ 100 ng/ml for > 48 h, while immunoreactive FSH concentrations were at baseline. The proportion of oocytes resuming meiosis and undergoing in-vitro fertilization (IVF) were comparable for r-hFSH (89%; 47 ± 19%) and r-HCG (88%; 50 ± 17%). In-vitro progesterone production and expression of progesterone receptors in granulosa cells did not differ between groups. Peak concentrations of serum progesterone in the luteal phase were similar, but were lower 6-9 days post-FSH relative to HCG. Thus, a bolus of r-hFSH was equivalent to r-HCG for the reinitiation of oocyte meiosis, fertilization and granulosa cell luteinization, but a midcycle FSH surge did not sustain normal luteal function in primates.

Original languageEnglish (US)
Pages (from-to)554-560
Number of pages7
JournalHuman Reproduction
Volume13
Issue number3
DOIs
StatePublished - 1998
Externally publishedYes

Keywords

  • Gonadotrophin surge
  • In-vitro fertilization
  • Oocyte maturation
  • Recombinant FSH
  • Recombinant HCG

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Fingerprint

Dive into the research topics of 'A bolus of recombinant human follicle stimulating hormone at midcycle induces periovulatory events following multiple follicular development in macaques'. Together they form a unique fingerprint.

Cite this