A bolus of recombinant human follicle stimulating hormone at midcycle induces periovulatory events following multiple follicular development in macaques

M. B. Zelinski-Wooten, J. S. Hutchison, D. L. Hess, D. P. WoIf, R. L. Stouffer

    Research output: Contribution to journalArticle

    31 Scopus citations

    Abstract

    The efficacy of follicle stimulating hormone (FSH) as an alternative to luteinizing hormone (LH)/human chorionic gonadotrophin (HCG) for the initiation of periovulatory events in primate follicles is unknown. A single bolus of 2500 IU recombinant (r)-hFSH was compared to 1000 IU r-HCG for its ability to promote oocyte nuclear maturation and fertilization, granulosa cell luteinization and corpus luteum function following r-hFSH (60 IU/day) induction of multiple follicular development in rhesus monkeys. Following the r-hFSH bolus, bioactive luteinizing hormone concentrations were < 3 ng/ml. Peak concentrations of serum FSH (1455 ± 314 mIU/ml; mean ± SEM) were attained 2-8 h after r-hFSH, and declined by 96 h. Bioactive HCG concentrations peaked between 2-8 h after r-HCG and remained ≤ 100 ng/ml for > 48 h, while immunoreactive FSH concentrations were at baseline. The proportion of oocytes resuming meiosis and undergoing in-vitro fertilization (IVF) were comparable for r-hFSH (89%; 47 ± 19%) and r-HCG (88%; 50 ± 17%). In-vitro progesterone production and expression of progesterone receptors in granulosa cells did not differ between groups. Peak concentrations of serum progesterone in the luteal phase were similar, but were lower 6-9 days post-FSH relative to HCG. Thus, a bolus of r-hFSH was equivalent to r-HCG for the reinitiation of oocyte meiosis, fertilization and granulosa cell luteinization, but a midcycle FSH surge did not sustain normal luteal function in primates.

    Original languageEnglish (US)
    Pages (from-to)554-560
    Number of pages7
    JournalHuman Reproduction
    Volume13
    Issue number3
    DOIs
    StatePublished - 1998

    Keywords

    • Gonadotrophin surge
    • In-vitro fertilization
    • Oocyte maturation
    • Recombinant FSH
    • Recombinant HCG

    ASJC Scopus subject areas

    • Reproductive Medicine
    • Obstetrics and Gynecology

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