TY - JOUR
T1 - A basic fuchsin and alkalinized methylene blue rapid stain for epoxyembedded tissue
AU - Huber, James D.
AU - Parker, Frank
AU - Odland, George F.
PY - 1968
Y1 - 1968
N2 - Sections 1 μ thick of epoxy-embedded, OsO4-fixed tissues were stained with 4% aqueous basic fuchsin at 70 C for 1 min, rinsed well and destained, also at 70 C, for 1 min. A 2% aqueous methylene blue solution, alkalinized to pH 12.5 by mixing 1 N NaOH with the dye on the slide in the proportion of about 2:1, was then allowed to act for 2 min at 23-27 C. The stain was rinsed off the slide, and the preparation air dried before applying a mounting medium and cover glass. The mounting medium consisted of immersion oil sealed with epoxy household cement. Stains had not faded after 1 yr. The method is simple, rapid (total time 4-5 min), and provides sharp contrast between cellular and connective tissue components.
AB - Sections 1 μ thick of epoxy-embedded, OsO4-fixed tissues were stained with 4% aqueous basic fuchsin at 70 C for 1 min, rinsed well and destained, also at 70 C, for 1 min. A 2% aqueous methylene blue solution, alkalinized to pH 12.5 by mixing 1 N NaOH with the dye on the slide in the proportion of about 2:1, was then allowed to act for 2 min at 23-27 C. The stain was rinsed off the slide, and the preparation air dried before applying a mounting medium and cover glass. The mounting medium consisted of immersion oil sealed with epoxy household cement. Stains had not faded after 1 yr. The method is simple, rapid (total time 4-5 min), and provides sharp contrast between cellular and connective tissue components.
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U2 - 10.3109/10520296809115048
DO - 10.3109/10520296809115048
M3 - Article
C2 - 4172336
AN - SCOPUS:84907129158
SN - 1052-0295
VL - 43
SP - 83
EP - 87
JO - Biotechnic and Histochemistry
JF - Biotechnic and Histochemistry
IS - 2
ER -