A basic fuchsin and alkalinized methylene blue rapid stain for epoxyembedded tissue

James D. Huber, Frank Parker, George F. Odland

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Sections 1 μ thick of epoxy-embedded, OsO4-fixed tissues were stained with 4% aqueous basic fuchsin at 70 C for 1 min, rinsed well and destained, also at 70 C, for 1 min. A 2% aqueous methylene blue solution, alkalinized to pH 12.5 by mixing 1 N NaOH with the dye on the slide in the proportion of about 2:1, was then allowed to act for 2 min at 23-27 C. The stain was rinsed off the slide, and the preparation air dried before applying a mounting medium and cover glass. The mounting medium consisted of immersion oil sealed with epoxy household cement. Stains had not faded after 1 yr. The method is simple, rapid (total time 4-5 min), and provides sharp contrast between cellular and connective tissue components.

Original languageEnglish (US)
Pages (from-to)83-87
Number of pages5
JournalBiotechnic and Histochemistry
Volume43
Issue number2
DOIs
StatePublished - 1968
Externally publishedYes

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Methylene Blue
Coloring Agents
Immersion
Connective Tissue
Glass
Oils
Air
basic fuchsin

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

Cite this

A basic fuchsin and alkalinized methylene blue rapid stain for epoxyembedded tissue. / Huber, James D.; Parker, Frank; Odland, George F.

In: Biotechnic and Histochemistry, Vol. 43, No. 2, 1968, p. 83-87.

Research output: Contribution to journalArticle

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