A basic fuchsin and alkalinized methylene blue rapid stain for epoxyembedded tissue

James D. Huber, Frank Parker, George F. Odland

Research output: Contribution to journalArticle

115 Scopus citations

Abstract

Sections 1 μ thick of epoxy-embedded, OsO4-fixed tissues were stained with 4% aqueous basic fuchsin at 70 C for 1 min, rinsed well and destained, also at 70 C, for 1 min. A 2% aqueous methylene blue solution, alkalinized to pH 12.5 by mixing 1 N NaOH with the dye on the slide in the proportion of about 2:1, was then allowed to act for 2 min at 23-27 C. The stain was rinsed off the slide, and the preparation air dried before applying a mounting medium and cover glass. The mounting medium consisted of immersion oil sealed with epoxy household cement. Stains had not faded after 1 yr. The method is simple, rapid (total time 4-5 min), and provides sharp contrast between cellular and connective tissue components.

Original languageEnglish (US)
Pages (from-to)83-87
Number of pages5
JournalBiotechnic and Histochemistry
Volume43
Issue number2
DOIs
StatePublished - Jan 1 1968

ASJC Scopus subject areas

  • Histology
  • Medical Laboratory Technology

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