A bacterial cell that synthesizes a protein containing the antigenic determinants of rat prolactin

Christopher R. Erwin, Richard Maurer, John E. Donelson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Bacterial minicells containing three different recombinant plasmids with rat prolactin cDNA sequences inserted at the Pst I site of pBR322 via the poly(dG):poly(dC) joining technique were examined for the expression of rat prolactin antigenic determinants. The three prolactin coding sequences were in the same orientation as the coding sequence of the ampicinin-resistance gene of pBR322. The presence of each of the three recombinant plasmids induced some prolactin synthesis by the bacteria as measured by imnunoprecipitation with anti-prolactin antisera. About 10% of the protein synthesized from one of the plasmids, prl 3, precipitated with the antisera. These prolactin antigenic determinants were part of a larger fused protein.

Original languageEnglish (US)
Pages (from-to)2537-2546
Number of pages10
JournalNucleic Acids Research
Volume8
Issue number11
DOIs
StatePublished - Jun 11 1980
Externally publishedYes

Fingerprint

Prolactin
Rats
Epitopes
Determinant
Proteins
Protein
Cell
Coding
Joining
Bacteria
Plasmids
Genes
CDNA
Immune Sera
Synthesis
Gene
Rat
Complementary DNA
Recombinant
Resistance

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

A bacterial cell that synthesizes a protein containing the antigenic determinants of rat prolactin. / Erwin, Christopher R.; Maurer, Richard; Donelson, John E.

In: Nucleic Acids Research, Vol. 8, No. 11, 11.06.1980, p. 2537-2546.

Research output: Contribution to journalArticle

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