A bacterial cell that synthesizes a protein containing the antigenic determinants of rat prolactin

Christopher R. Erwin, Richard Maurer, John E. Donelson

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3 Scopus citations

Abstract

Bacterial minicells containing three different recombinant plasmids with rat prolactin cDNA sequences inserted at the Pst I site of pBR322 via the poly(dG):poly(dC) joining technique were examined for the expression of rat prolactin antigenic determinants. The three prolactin coding sequences were in the same orientation as the coding sequence of the ampicinin-resistance gene of pBR322. The presence of each of the three recombinant plasmids induced some prolactin synthesis by the bacteria as measured by imnunoprecipitation with anti-prolactin antisera. About 10% of the protein synthesized from one of the plasmids, prl 3, precipitated with the antisera. These prolactin antigenic determinants were part of a larger fused protein.

Original languageEnglish (US)
Pages (from-to)2537-2546
Number of pages10
JournalNucleic Acids Research
Volume8
Issue number11
DOIs
Publication statusPublished - Jun 11 1980
Externally publishedYes

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ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

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