A bacterial cell that synthesizes a protein containing the antigenic determinants of rat prolactin

Christopher R. Erwin; Richard A. Maurer; John E. Donelson

doi:10.1093/nar/8.11.2537

A bacterial cell that synthesizes a protein containing the antigenic determinants of rat prolactin

Christopher R. Erwin, Richard A. Maurer, John E. Donelson

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Bacterial minicells containing three different recombinant plasmids with rat prolactin cDNA sequences inserted at the Pst I site of pBR322 via the poly(dG):poly(dC) joining technique were examined for the expression of rat prolactin antigenic determinants. The three prolactin coding sequences were in the same orientation as the coding sequence of the ampicinin-resistance gene of pBR322. The presence of each of the three recombinant plasmids induced some prolactin synthesis by the bacteria as measured by imnunoprecipitation with anti-prolactin antisera. About 10% of the protein synthesized from one of the plasmids, prl 3, precipitated with the antisera. These prolactin antigenic determinants were part of a larger fused protein.

Original language	English (US)
Pages (from-to)	2537-2546
Number of pages	10
Journal	Nucleic acids research
Volume	8
Issue number	11
DOIs	https://doi.org/10.1093/nar/8.11.2537
State	Published - Jun 11 1980
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "A bacterial cell that synthesizes a protein containing the antigenic determinants of rat prolactin",

abstract = "Bacterial minicells containing three different recombinant plasmids with rat prolactin cDNA sequences inserted at the Pst I site of pBR322 via the poly(dG):poly(dC) joining technique were examined for the expression of rat prolactin antigenic determinants. The three prolactin coding sequences were in the same orientation as the coding sequence of the ampicinin-resistance gene of pBR322. The presence of each of the three recombinant plasmids induced some prolactin synthesis by the bacteria as measured by imnunoprecipitation with anti-prolactin antisera. About 10% of the protein synthesized from one of the plasmids, prl 3, precipitated with the antisera. These prolactin antigenic determinants were part of a larger fused protein.",

author = "Erwin, {Christopher R.} and Maurer, {Richard A.} and Donelson, {John E.}",

note = "Funding Information: ACKNOWLEDGMENTS This research was supported by NSF grant PCM 76-13451 to J.E.D. and NIH grant AM 21803 to R.A.M. C.R.E. was supported by NIH predoctoral genetics training grant GM 07091.",

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T1 - A bacterial cell that synthesizes a protein containing the antigenic determinants of rat prolactin

AU - Erwin, Christopher R.

AU - Maurer, Richard A.

AU - Donelson, John E.

N1 - Funding Information: ACKNOWLEDGMENTS This research was supported by NSF grant PCM 76-13451 to J.E.D. and NIH grant AM 21803 to R.A.M. C.R.E. was supported by NIH predoctoral genetics training grant GM 07091.

PY - 1980/6/11

Y1 - 1980/6/11

N2 - Bacterial minicells containing three different recombinant plasmids with rat prolactin cDNA sequences inserted at the Pst I site of pBR322 via the poly(dG):poly(dC) joining technique were examined for the expression of rat prolactin antigenic determinants. The three prolactin coding sequences were in the same orientation as the coding sequence of the ampicinin-resistance gene of pBR322. The presence of each of the three recombinant plasmids induced some prolactin synthesis by the bacteria as measured by imnunoprecipitation with anti-prolactin antisera. About 10% of the protein synthesized from one of the plasmids, prl 3, precipitated with the antisera. These prolactin antigenic determinants were part of a larger fused protein.

AB - Bacterial minicells containing three different recombinant plasmids with rat prolactin cDNA sequences inserted at the Pst I site of pBR322 via the poly(dG):poly(dC) joining technique were examined for the expression of rat prolactin antigenic determinants. The three prolactin coding sequences were in the same orientation as the coding sequence of the ampicinin-resistance gene of pBR322. The presence of each of the three recombinant plasmids induced some prolactin synthesis by the bacteria as measured by imnunoprecipitation with anti-prolactin antisera. About 10% of the protein synthesized from one of the plasmids, prl 3, precipitated with the antisera. These prolactin antigenic determinants were part of a larger fused protein.

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A bacterial cell that synthesizes a protein containing the antigenic determinants of rat prolactin

Abstract

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