A 76-bp deletion in the Mip gene causes autosomal dominant cataract in Hfi mice

D. J. Sidjanin, Devonne M. Parker-Wilson, Angelika Neuhäuser-Klaus, Walter Pretsch, Jack Favor, Peter M.T. Deen, Chiaki Ohtaka-Maruyama, Yun Lu, Alvina Bragin, William R. Skach, Ana B. Chepelinsky, Patricia A. Grimes, Dwight E. Stambolian

    Research output: Contribution to journalArticlepeer-review

    38 Scopus citations

    Abstract

    Hfi is a dominant cataract mutation where heterozygotes show hydropic lens fibers and homozygotes show total lens opacity. The Hfi locus was mapped to the distal part of mouse chromosome 10 close to the major intrinsic protein (Mip), which is expressed only in cell membranes of lens fibers. Molecular analysis of Mip revealed a 76-bp deletion that resulted in exon 2 skipping in Mip mRNA. In Hfi/Hfi this deletion resulted in a complete absence of the wildtype Mip. In contrast, Hfi/+ animals had the same amount of wildtype Mip as +/+. Results from pulse-chase expression studies excluded hetero-oligomerization of wildtype and mutant Mip as a possible mechanism for cataract formation in the Hfi/+. We propose that the cataract phenotype in the Hfi heterozygote mutant is due to a detrimental gain of function by the mutant Mip resulting in either cytotoxicity or disruption in processing of other proteins important for the lens. Cataract formation in the Hfi/Hfi mouse is probably a combined result of both the complete loss of wildtype Mip and a gain of function of the mutant Mip.

    Original languageEnglish (US)
    Pages (from-to)313-319
    Number of pages7
    JournalGenomics
    Volume74
    Issue number3
    DOIs
    StatePublished - Jun 15 2001

    ASJC Scopus subject areas

    • Genetics

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