A 24-base-pair DNA sequence from the MAT locus stimulates intergenic recombination in yeast

J. A. Nickoloff, E. Y. Chen, F. Heffron

Research output: Contribution to journalArticlepeer-review

118 Scopus citations

Abstract

HO nuclease is a site-specific double-strand endonuclease present in haploid Saccharomyces cerevisiae undergoing mating type interconversion. HO nucleus initiates mating type interconversion by making a double-strand break within the MAT locus. To define the recognition site for the enzyme in vitro, we have constructed a number of point mutations and deletions within or adjacent to the HO recognition site. Digestion of these substrates with HO in vitro reveals that the minimal recognition site is 18 base pairs long, although several shorter substrates and substrates containing point mutations are cleaved at low levels in vitro. A 24-base-pair HO recognition site stimulates homologous recombination when present in a region unrelated to MAT. Recombinants arise from both gene conversion and crossover events. The identification of the HO recognition site provides a way of introducing a defined initiation site for recombination.

Original languageEnglish (US)
Pages (from-to)7831-7835
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number20
DOIs
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • General

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