A γδ+ T-cell leukemia bearing a novel t(8;14) (q24;q11) translocation demonstrates spontaneous in vitro natural killer-like activity

Richard Maziarz, Robert J. Arceci, Shelly C. Bernstein, Lindsay Frazier, Brian R. Smith, Masataka Kasai, Ramana Tantravahi, Jack L. Strominger

Research output: Contribution to journalArticle

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Abstract

A highly malignant human T-cell leukemia was identified by cell surface analysis as a member of the T-cell receptor (TCR) γδ lineage. Cytogenetic and molecular analysis showed a novel t(8;14)(q24;q11) rearrangement involving the Jδ1 gene segment on chromosome 14 and the distal end of chromosome 8 near the c-myc proto-oncogene locus. The γδ TCR of the leukemia blasts was functionally intact and could be activated to generate intracellular calcium flux and to target Fc receptor-mediated redirected tumor cell lysis. In addition, non-major histocompatibility complex restricted lysis of a limited target cell panel was shown by fresh leukemic blasts and by the in vitro-maintained leukemia cells that was comparable to known T-cell lines with natural killer-like activity. These data suggest that the T-cell leukemia potentially had in vivo functional cytolytic activity. However, whether this activity did contribute to the patient's clinical condition could not be determined.

Original languageEnglish (US)
Pages (from-to)1523-1531
Number of pages9
JournalBlood
Volume79
Issue number6
StatePublished - Mar 15 1992

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Bearings (structural)
T-Cell Leukemia
T-cells
Chromosomes
T-Cell Antigen Receptor
Fc Receptors
Surface analysis
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 8
myc Genes
Tumors
Cytogenetic Analysis
Cell Lineage
Genes
Major Histocompatibility Complex
Cells
Fluxes
Calcium
Leukemia
T-Lymphocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Maziarz, R., Arceci, R. J., Bernstein, S. C., Frazier, L., Smith, B. R., Kasai, M., ... Strominger, J. L. (1992). A γδ+ T-cell leukemia bearing a novel t(8;14) (q24;q11) translocation demonstrates spontaneous in vitro natural killer-like activity. Blood, 79(6), 1523-1531.

A γδ+ T-cell leukemia bearing a novel t(8;14) (q24;q11) translocation demonstrates spontaneous in vitro natural killer-like activity. / Maziarz, Richard; Arceci, Robert J.; Bernstein, Shelly C.; Frazier, Lindsay; Smith, Brian R.; Kasai, Masataka; Tantravahi, Ramana; Strominger, Jack L.

In: Blood, Vol. 79, No. 6, 15.03.1992, p. 1523-1531.

Research output: Contribution to journalArticle

Maziarz, R, Arceci, RJ, Bernstein, SC, Frazier, L, Smith, BR, Kasai, M, Tantravahi, R & Strominger, JL 1992, 'A γδ+ T-cell leukemia bearing a novel t(8;14) (q24;q11) translocation demonstrates spontaneous in vitro natural killer-like activity', Blood, vol. 79, no. 6, pp. 1523-1531.
Maziarz, Richard ; Arceci, Robert J. ; Bernstein, Shelly C. ; Frazier, Lindsay ; Smith, Brian R. ; Kasai, Masataka ; Tantravahi, Ramana ; Strominger, Jack L. / A γδ+ T-cell leukemia bearing a novel t(8;14) (q24;q11) translocation demonstrates spontaneous in vitro natural killer-like activity. In: Blood. 1992 ; Vol. 79, No. 6. pp. 1523-1531.
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abstract = "A highly malignant human T-cell leukemia was identified by cell surface analysis as a member of the T-cell receptor (TCR) γδ lineage. Cytogenetic and molecular analysis showed a novel t(8;14)(q24;q11) rearrangement involving the Jδ1 gene segment on chromosome 14 and the distal end of chromosome 8 near the c-myc proto-oncogene locus. The γδ TCR of the leukemia blasts was functionally intact and could be activated to generate intracellular calcium flux and to target Fc receptor-mediated redirected tumor cell lysis. In addition, non-major histocompatibility complex restricted lysis of a limited target cell panel was shown by fresh leukemic blasts and by the in vitro-maintained leukemia cells that was comparable to known T-cell lines with natural killer-like activity. These data suggest that the T-cell leukemia potentially had in vivo functional cytolytic activity. However, whether this activity did contribute to the patient's clinical condition could not be determined.",
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AU - Strominger, Jack L.

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