[9] Receptor expression in mammalian cells

Rachael L. Neve, Kim A. Neve

Research output: Research - peer-reviewArticle

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Abstract

The methods outlined above for the cloning of dopamine receptor cDNAs and their expression in mammalian cells allow the investigator to study dopamine receptor pharmacology with a precision and detail not previously possible. These strategies can be used to create stable and clonal genetically engineered cell lines that express a given receptor subtype, a specific spliced form of a receptor subtype, or mutant and chimeric receptors. Such transfected cells can be evaluated to determine how receptors bind ligands in a receptor-selective manner, to learn how receptors transduce the energy of the binding of agonists into a functional response, to ascertain the mechanisms by which specificity or selectivity of coupling of receptors to various G proteins and signaling pathways is attained, and to reveal the molecular basis of regulation of receptor sensitivity.

LanguageEnglish (US)
Pages163-174
Number of pages12
JournalMethods in Neurosciences
Volume25
Issue numberC
DOIs
StatePublished - 1995

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Dopamine Receptors
GTP-Binding Proteins
Organism Cloning
Complementary DNA
Research Personnel
Pharmacology
Ligands
Cell Line

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

[9] Receptor expression in mammalian cells. / Neve, Rachael L.; Neve, Kim A.

In: Methods in Neurosciences, Vol. 25, No. C, 1995, p. 163-174.

Research output: Research - peer-reviewArticle

Neve, Rachael L. ; Neve, Kim A./ [9] Receptor expression in mammalian cells. In: Methods in Neurosciences. 1995 ; Vol. 25, No. C. pp. 163-174
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