5-hydroxytryptamine(1B) receptors block the GABA(B) synaptic potential in rat dopamine neurons

Steven Johnson, N. B. Mercuri, R. A. North

Research output: Contribution to journalArticle

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Abstract

Intracellular recordings were made from presumed dopamine-containing neurons in slices cut from the midbrain of the rat. Focal electrical stimulation produced a hyperpolarizing synaptic potential that was reduced by 75-95% by the GABA(B)-receptor antagonist 2-hydroxysaclofen (300 μM). 5-HT (3-100 μM) reduced the amplitude of the GABA(B) synaptic potential by 20- 74%, with a 50% reduction at 10 μM, but did not reduce the amplitude of synaptic potentials mediated by GABA(A) receptors. 5-HT acted presynaptically because hyperpolarizations produced by exogenously administered GABA (1 mM) in picrotoxin (100 μM) were not affected by 5-HT (30 μM). (±)- Cyanopindolol (100 nM), a 5-HT(1B) antagonist, blocked the effect of 5-HT (10 μM); spiperone (1 μM), which is an antagonist at 5-HT(1A) and 5-HT2 receptors, had no effect. The amplitude of the GABA(B) synaptic potential was reduced by the 5-HT(1B) receptor agonists 1-[3-(trifluoromethyl)-phenyl]- piperazine (300 nM) and 7-trifluoromethyl-4-(4-methyl-1-piperazinyl)- pyrrolo[1,2-a]quinoxaline (1 μM), but not by the 5-HT(1A) agonist N,N- dipropyl-5-carboxamidotryptamine (1 μM) or the 5-HT2 agonist (±)-1-(2,5- dimethoxy-4-iodophenyl)-2-amino-propane (10 μM). We conclude that 5-HT activates presynaptic 5-HT(1B) receptors that inhibit the release of GABA onto GABA(B) but not GABA(A) receptors.

Original languageEnglish (US)
Pages (from-to)2000-2006
Number of pages7
JournalJournal of Neuroscience
Volume12
Issue number5
StatePublished - 1992

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Receptor, Serotonin, 5-HT1B
Synaptic Potentials
Dopaminergic Neurons
gamma-Aminobutyric Acid
Serotonin
GABA-A Receptors
GABA-B Receptor Antagonists
Serotonin 5-HT2 Receptor Agonists
Spiperone
Serotonin Receptor Agonists
Picrotoxin
Propane
Serotonin Antagonists
Receptor, Serotonin, 5-HT1A
Mesencephalon
Electric Stimulation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

5-hydroxytryptamine(1B) receptors block the GABA(B) synaptic potential in rat dopamine neurons. / Johnson, Steven; Mercuri, N. B.; North, R. A.

In: Journal of Neuroscience, Vol. 12, No. 5, 1992, p. 2000-2006.

Research output: Contribution to journalArticle

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