5-HT-Mediated synaptic potentials in the dorsal raphe nucleus

Interactions with excitatory amino acid and GABA neurotransmission

Z. Z. Pan, W. F. Colmers, John Williams

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Intracellular recordings from neurons within dorsal raphe nucleus in slices from rat brain were used to study an inhibitory postsynaptic potential (IPSP) evoked by electrical stimulation. The IPSP was observed in ~70% of neurons, had a latency to onset of 40-65 ms, reached a peak in 350-400 ms, had a total duration of 1-2 s, and reversed polarity at the potassium equilibrium potential. This IPSP was blocked by spiperone (1 μM) and prolonged by fluoxetine (300 nM - 30 μM) suggesting that it was mediated by 5-hydroxytryptamine (5-HT). Superfusion with γ-aminobutyric acid (GABA) and excitatory amino acid receptor antagonists were used to block 'fast' synaptic potentials that preceded the IPSP such that it could be studied in isolation. Blockade of the GABA-mediated synaptic potentials increased the amplitude of the IPSP by 1.3-fold. The amplitude of the IPSP was reduced by 30% after blockade of the excitatory amino acid-mediated synaptic potential. The results indicate that the IPSP recorded in dorsal raphe neurons was caused by 5-HT released at least in part from indirect (synaptically induced) excitation of 5-HT-containing cells within the slice.

Original languageEnglish (US)
Pages (from-to)481-486
Number of pages6
JournalJournal of Neurophysiology
Volume62
Issue number2
StatePublished - 1989

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Inhibitory Postsynaptic Potentials
Synaptic Potentials
Excitatory Amino Acids
Synaptic Transmission
gamma-Aminobutyric Acid
Serotonin
Neurons
Aminobutyrates
Spiperone
Excitatory Amino Acid Antagonists
Fluoxetine
Glutamate Receptors
Dorsal Raphe Nucleus
Electric Stimulation
Potassium
Brain

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

5-HT-Mediated synaptic potentials in the dorsal raphe nucleus : Interactions with excitatory amino acid and GABA neurotransmission. / Pan, Z. Z.; Colmers, W. F.; Williams, John.

In: Journal of Neurophysiology, Vol. 62, No. 2, 1989, p. 481-486.

Research output: Contribution to journalArticle

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