5-HT inhibits synaptic transmission in rat subthalamic nucleus neurons in vitro

K. Z. Shen; S. W. Johnson

doi:10.1016/j.neuroscience.2007.12.001

5-HT inhibits synaptic transmission in rat subthalamic nucleus neurons in vitro

K. Z. Shen, S. W. Johnson

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

The subthalamic nucleus (STN) plays a pivotal role in normal and abnormal motor function. We used patch pipettes to study effects of 5-HT on synaptic currents evoked in STN neurons by focal electrical stimulation of rat brain slices. 5-HT (10 μM) reduced glutamate-mediated excitatory postsynaptic currents (EPSCs) by 35±4%. However, a much higher concentration of 5-HT (100 μM) was required to inhibit GABA-mediated inhibitory postsynaptic currents (IPSCs) to a comparable extent. Concentration-response curves showed that the 5-HT inhibitory concentration 50% (IC₅₀) for inhibition of IPSCs (20.2 μM) was more than fivefold greater than the IC₅₀ for inhibition of EPSCs (3.4 μM). The 5-HT-induced reductions in EPSCs and IPSCs were accompanied by increases in paired-pulse ratios, indicating that 5-HT acts presynaptically to inhibit synaptic transmission. The 5-HT_1B receptor antagonist NAS-181 significantly antagonized 5-HT-induced inhibitions of EPSCs and IPSCs. These studies show that 5-HT inhibits synaptic transmission in the STN by activating presynaptic 5-HT_1B receptors.

Original language	English (US)
Pages (from-to)	1029-1033
Number of pages	5
Journal	Neuroscience
Volume	151
Issue number	4
DOIs	https://doi.org/10.1016/j.neuroscience.2007.12.001
State	Published - Feb 19 2008
Externally published	Yes

Keywords

5-HT
EPSC
IPSC
brain slice
patch clamp
presynaptic inhibition

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neuroscience.2007.12.001

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title = "5-HT inhibits synaptic transmission in rat subthalamic nucleus neurons in vitro",

abstract = "The subthalamic nucleus (STN) plays a pivotal role in normal and abnormal motor function. We used patch pipettes to study effects of 5-HT on synaptic currents evoked in STN neurons by focal electrical stimulation of rat brain slices. 5-HT (10 μM) reduced glutamate-mediated excitatory postsynaptic currents (EPSCs) by 35±4%. However, a much higher concentration of 5-HT (100 μM) was required to inhibit GABA-mediated inhibitory postsynaptic currents (IPSCs) to a comparable extent. Concentration-response curves showed that the 5-HT inhibitory concentration 50% (IC50) for inhibition of IPSCs (20.2 μM) was more than fivefold greater than the IC50 for inhibition of EPSCs (3.4 μM). The 5-HT-induced reductions in EPSCs and IPSCs were accompanied by increases in paired-pulse ratios, indicating that 5-HT acts presynaptically to inhibit synaptic transmission. The 5-HT1B receptor antagonist NAS-181 significantly antagonized 5-HT-induced inhibitions of EPSCs and IPSCs. These studies show that 5-HT inhibits synaptic transmission in the STN by activating presynaptic 5-HT1B receptors.",

keywords = "5-HT, EPSC, IPSC, brain slice, patch clamp, presynaptic inhibition",

author = "Shen, {K. Z.} and Johnson, {S. W.}",

note = "Funding Information: This study was supported by USPHS grant NS38175 and by the Portland Veterans Affairs Parkinson{\textquoteright}s Disease Research, Education, and Clinical Center.",

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AU - Johnson, S. W.

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N2 - The subthalamic nucleus (STN) plays a pivotal role in normal and abnormal motor function. We used patch pipettes to study effects of 5-HT on synaptic currents evoked in STN neurons by focal electrical stimulation of rat brain slices. 5-HT (10 μM) reduced glutamate-mediated excitatory postsynaptic currents (EPSCs) by 35±4%. However, a much higher concentration of 5-HT (100 μM) was required to inhibit GABA-mediated inhibitory postsynaptic currents (IPSCs) to a comparable extent. Concentration-response curves showed that the 5-HT inhibitory concentration 50% (IC50) for inhibition of IPSCs (20.2 μM) was more than fivefold greater than the IC50 for inhibition of EPSCs (3.4 μM). The 5-HT-induced reductions in EPSCs and IPSCs were accompanied by increases in paired-pulse ratios, indicating that 5-HT acts presynaptically to inhibit synaptic transmission. The 5-HT1B receptor antagonist NAS-181 significantly antagonized 5-HT-induced inhibitions of EPSCs and IPSCs. These studies show that 5-HT inhibits synaptic transmission in the STN by activating presynaptic 5-HT1B receptors.

AB - The subthalamic nucleus (STN) plays a pivotal role in normal and abnormal motor function. We used patch pipettes to study effects of 5-HT on synaptic currents evoked in STN neurons by focal electrical stimulation of rat brain slices. 5-HT (10 μM) reduced glutamate-mediated excitatory postsynaptic currents (EPSCs) by 35±4%. However, a much higher concentration of 5-HT (100 μM) was required to inhibit GABA-mediated inhibitory postsynaptic currents (IPSCs) to a comparable extent. Concentration-response curves showed that the 5-HT inhibitory concentration 50% (IC50) for inhibition of IPSCs (20.2 μM) was more than fivefold greater than the IC50 for inhibition of EPSCs (3.4 μM). The 5-HT-induced reductions in EPSCs and IPSCs were accompanied by increases in paired-pulse ratios, indicating that 5-HT acts presynaptically to inhibit synaptic transmission. The 5-HT1B receptor antagonist NAS-181 significantly antagonized 5-HT-induced inhibitions of EPSCs and IPSCs. These studies show that 5-HT inhibits synaptic transmission in the STN by activating presynaptic 5-HT1B receptors.

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KW - IPSC

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KW - patch clamp

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5-HT inhibits synaptic transmission in rat subthalamic nucleus neurons in vitro

Abstract

Keywords

ASJC Scopus subject areas

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