23Na-NMR measurements of intracellular sodium in intact perfused ferret hearts during ischemia and reperfusion

Martin Pike, M. Kitakaze, E. Marban

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134 Citations (Scopus)

Abstract

23Na nuclear magnetic resonance (NMR) spectroscopy was utilized to measure intracellular Na+ in perfused ferret hearts exposed to the shift reagent dysprosium triethylenetetramine-hexa-acetic acid [Dy(TTHA)3-]. The intracellular Na+ signal was small under normal perfusion conditions; resolution was enhanced by using a Jump-Return NMR pulse protocol. During 20 min of total global ischemia at 30°C, intracellular Na+ concentration ([Na+](i)) increased steadily to a peak value fivefold greater than control. [Na+](i) declined monotonically back to control levels within 9 min of reperfusion. In contrast, the mean contractile pressure only recovered to 54% of control levels. Thus major alterations in Na+ homeostasis occur during severe ischemia. [Na+] recovers rapidly during reperfusion and is therefore dissociated from the lingering postischemic depression of contractile function known as 'stunning'.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume259
Issue number6 28-6
StatePublished - 1990
Externally publishedYes

Fingerprint

Ferrets
Reperfusion
Dysprosium
Magnetic Resonance Spectroscopy
Ischemia
Sodium
Trientine
Acetic Acid
Homeostasis
Perfusion
Pressure
triethylenetetraminehexaacetic acid

Keywords

  • cardiac function
  • nuclear magnetic resonance spectroscopy
  • stunned myocardium

ASJC Scopus subject areas

  • Physiology
  • Medicine(all)

Cite this

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abstract = "23Na nuclear magnetic resonance (NMR) spectroscopy was utilized to measure intracellular Na+ in perfused ferret hearts exposed to the shift reagent dysprosium triethylenetetramine-hexa-acetic acid [Dy(TTHA)3-]. The intracellular Na+ signal was small under normal perfusion conditions; resolution was enhanced by using a Jump-Return NMR pulse protocol. During 20 min of total global ischemia at 30°C, intracellular Na+ concentration ([Na+](i)) increased steadily to a peak value fivefold greater than control. [Na+](i) declined monotonically back to control levels within 9 min of reperfusion. In contrast, the mean contractile pressure only recovered to 54{\%} of control levels. Thus major alterations in Na+ homeostasis occur during severe ischemia. [Na+] recovers rapidly during reperfusion and is therefore dissociated from the lingering postischemic depression of contractile function known as 'stunning'.",
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AB - 23Na nuclear magnetic resonance (NMR) spectroscopy was utilized to measure intracellular Na+ in perfused ferret hearts exposed to the shift reagent dysprosium triethylenetetramine-hexa-acetic acid [Dy(TTHA)3-]. The intracellular Na+ signal was small under normal perfusion conditions; resolution was enhanced by using a Jump-Return NMR pulse protocol. During 20 min of total global ischemia at 30°C, intracellular Na+ concentration ([Na+](i)) increased steadily to a peak value fivefold greater than control. [Na+](i) declined monotonically back to control levels within 9 min of reperfusion. In contrast, the mean contractile pressure only recovered to 54% of control levels. Thus major alterations in Na+ homeostasis occur during severe ischemia. [Na+] recovers rapidly during reperfusion and is therefore dissociated from the lingering postischemic depression of contractile function known as 'stunning'.

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