1H-NMR spectroscopy can accurately quantitate the lipolysis and oxidation of cardiac triacylglycerols

Michael C. Madden, W. Barry Van Winkle, Katharine Kirk, Martin Pike, Gerald M. Pohost, Paul E. Wolkowicz

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Triacylglycerol metabolism in isolated, perfused hearts from rats fed a diet containing 20% rapeseed oil (RSO) was studied using 1H-NMR spectroscopy. RSO-induced elevation in cardiac triacylglycerols is associated with an increase in the peak area of fatty acid 1H-NMR resonances. The ratio of methyl, γ-methylene or methylene protons adjacent to a carbon-carbon double bond to the number of methylene protons in these hearts measured by 1H-NMR spectroscopy gives values similar to those derived from previously reported chemical analyses. In addition, the triacylglycerol content of these hearts determined by chemical analysis directly correlates with their content of 1H-NMR visible fatty acid resonances. This quantitative relationship allows the real-time measurement of the rates of cardiac triacylglycerol lipolysis using 1H-NMR spectroscopy. Rates of triacylglycerol lipolysis measured using 1H-NMR spectroscopy are similar to those previously measured by chemical methods. Triacylglycerol lipolysis measured using 1H-NMR spectroscopy occurs at a significantly faster rate in hearts perfused in the presence or absence of glucose when compared to hearts perfused with glucose and acetate or medium-chain fatty acids. Finally, the rate of triacylglycerol lipolysis in glucose perfused hearts is linearly related to work output. These results demonstrate that 1H-NMR spectroscopy can accurately quantitate triacylglycerol content and metabolism in the rapeseed oil-fed rat model. 1H-NMR spectroscopic or imaging techniques may be useful in the real-time evaluation of cardiac triacylglycerol content and metabolism.

Original languageEnglish (US)
Pages (from-to)176-182
Number of pages7
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1169
Issue number2
DOIs
StatePublished - Aug 11 1993
Externally publishedYes

Fingerprint

Lipolysis
Nuclear magnetic resonance spectroscopy
Triglycerides
Magnetic Resonance Spectroscopy
Oxidation
Metabolism
Fatty Acids
Nuclear magnetic resonance
Glucose
Protons
Rats
Carbon
Proton Magnetic Resonance Spectroscopy
Nutrition
Time measurement
Acetates
Heart Rate
Diet
Imaging techniques

Keywords

  • Cardiac
  • Fatty acid metabolism
  • Lipolysis
  • NMR, H-
  • Triacylglycerol

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology
  • Medicine(all)

Cite this

1H-NMR spectroscopy can accurately quantitate the lipolysis and oxidation of cardiac triacylglycerols. / Madden, Michael C.; Van Winkle, W. Barry; Kirk, Katharine; Pike, Martin; Pohost, Gerald M.; Wolkowicz, Paul E.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 1169, No. 2, 11.08.1993, p. 176-182.

Research output: Contribution to journalArticle

Madden, Michael C. ; Van Winkle, W. Barry ; Kirk, Katharine ; Pike, Martin ; Pohost, Gerald M. ; Wolkowicz, Paul E. / 1H-NMR spectroscopy can accurately quantitate the lipolysis and oxidation of cardiac triacylglycerols. In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1993 ; Vol. 1169, No. 2. pp. 176-182.
@article{155198c58dcd49b5ac1b995be2711847,
title = "1H-NMR spectroscopy can accurately quantitate the lipolysis and oxidation of cardiac triacylglycerols",
abstract = "Triacylglycerol metabolism in isolated, perfused hearts from rats fed a diet containing 20{\%} rapeseed oil (RSO) was studied using 1H-NMR spectroscopy. RSO-induced elevation in cardiac triacylglycerols is associated with an increase in the peak area of fatty acid 1H-NMR resonances. The ratio of methyl, γ-methylene or methylene protons adjacent to a carbon-carbon double bond to the number of methylene protons in these hearts measured by 1H-NMR spectroscopy gives values similar to those derived from previously reported chemical analyses. In addition, the triacylglycerol content of these hearts determined by chemical analysis directly correlates with their content of 1H-NMR visible fatty acid resonances. This quantitative relationship allows the real-time measurement of the rates of cardiac triacylglycerol lipolysis using 1H-NMR spectroscopy. Rates of triacylglycerol lipolysis measured using 1H-NMR spectroscopy are similar to those previously measured by chemical methods. Triacylglycerol lipolysis measured using 1H-NMR spectroscopy occurs at a significantly faster rate in hearts perfused in the presence or absence of glucose when compared to hearts perfused with glucose and acetate or medium-chain fatty acids. Finally, the rate of triacylglycerol lipolysis in glucose perfused hearts is linearly related to work output. These results demonstrate that 1H-NMR spectroscopy can accurately quantitate triacylglycerol content and metabolism in the rapeseed oil-fed rat model. 1H-NMR spectroscopic or imaging techniques may be useful in the real-time evaluation of cardiac triacylglycerol content and metabolism.",
keywords = "Cardiac, Fatty acid metabolism, Lipolysis, NMR, H-, Triacylglycerol",
author = "Madden, {Michael C.} and {Van Winkle}, {W. Barry} and Katharine Kirk and Martin Pike and Pohost, {Gerald M.} and Wolkowicz, {Paul E.}",
year = "1993",
month = "8",
day = "11",
doi = "10.1016/0005-2760(93)90203-L",
language = "English (US)",
volume = "1169",
pages = "176--182",
journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids",
issn = "1388-1981",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - 1H-NMR spectroscopy can accurately quantitate the lipolysis and oxidation of cardiac triacylglycerols

AU - Madden, Michael C.

AU - Van Winkle, W. Barry

AU - Kirk, Katharine

AU - Pike, Martin

AU - Pohost, Gerald M.

AU - Wolkowicz, Paul E.

PY - 1993/8/11

Y1 - 1993/8/11

N2 - Triacylglycerol metabolism in isolated, perfused hearts from rats fed a diet containing 20% rapeseed oil (RSO) was studied using 1H-NMR spectroscopy. RSO-induced elevation in cardiac triacylglycerols is associated with an increase in the peak area of fatty acid 1H-NMR resonances. The ratio of methyl, γ-methylene or methylene protons adjacent to a carbon-carbon double bond to the number of methylene protons in these hearts measured by 1H-NMR spectroscopy gives values similar to those derived from previously reported chemical analyses. In addition, the triacylglycerol content of these hearts determined by chemical analysis directly correlates with their content of 1H-NMR visible fatty acid resonances. This quantitative relationship allows the real-time measurement of the rates of cardiac triacylglycerol lipolysis using 1H-NMR spectroscopy. Rates of triacylglycerol lipolysis measured using 1H-NMR spectroscopy are similar to those previously measured by chemical methods. Triacylglycerol lipolysis measured using 1H-NMR spectroscopy occurs at a significantly faster rate in hearts perfused in the presence or absence of glucose when compared to hearts perfused with glucose and acetate or medium-chain fatty acids. Finally, the rate of triacylglycerol lipolysis in glucose perfused hearts is linearly related to work output. These results demonstrate that 1H-NMR spectroscopy can accurately quantitate triacylglycerol content and metabolism in the rapeseed oil-fed rat model. 1H-NMR spectroscopic or imaging techniques may be useful in the real-time evaluation of cardiac triacylglycerol content and metabolism.

AB - Triacylglycerol metabolism in isolated, perfused hearts from rats fed a diet containing 20% rapeseed oil (RSO) was studied using 1H-NMR spectroscopy. RSO-induced elevation in cardiac triacylglycerols is associated with an increase in the peak area of fatty acid 1H-NMR resonances. The ratio of methyl, γ-methylene or methylene protons adjacent to a carbon-carbon double bond to the number of methylene protons in these hearts measured by 1H-NMR spectroscopy gives values similar to those derived from previously reported chemical analyses. In addition, the triacylglycerol content of these hearts determined by chemical analysis directly correlates with their content of 1H-NMR visible fatty acid resonances. This quantitative relationship allows the real-time measurement of the rates of cardiac triacylglycerol lipolysis using 1H-NMR spectroscopy. Rates of triacylglycerol lipolysis measured using 1H-NMR spectroscopy are similar to those previously measured by chemical methods. Triacylglycerol lipolysis measured using 1H-NMR spectroscopy occurs at a significantly faster rate in hearts perfused in the presence or absence of glucose when compared to hearts perfused with glucose and acetate or medium-chain fatty acids. Finally, the rate of triacylglycerol lipolysis in glucose perfused hearts is linearly related to work output. These results demonstrate that 1H-NMR spectroscopy can accurately quantitate triacylglycerol content and metabolism in the rapeseed oil-fed rat model. 1H-NMR spectroscopic or imaging techniques may be useful in the real-time evaluation of cardiac triacylglycerol content and metabolism.

KW - Cardiac

KW - Fatty acid metabolism

KW - Lipolysis

KW - NMR, H-

KW - Triacylglycerol

UR - http://www.scopus.com/inward/record.url?scp=0027164644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027164644&partnerID=8YFLogxK

U2 - 10.1016/0005-2760(93)90203-L

DO - 10.1016/0005-2760(93)90203-L

M3 - Article

C2 - 8343541

AN - SCOPUS:0027164644

VL - 1169

SP - 176

EP - 182

JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 2

ER -