1H HR-MAS spectroscopy for quantitative measurement of choline concentration in amniotic fluid as a marker of fetal lung maturity

Interand intraobserver reproducibility study

Bonnie N. Joe, Kiarash Vahidi, Andrew Zektzer, Mei Hsiu Chen, Matthew S. Clifton, Thomas Butler, Kayvan Keshari, John Kurhanewicz, Fergus Coakley, Mark G. Swanson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Purpose: To determine the intra- and interobserver reproducibility of human amniotic fluid metabolite concentration measurements (including potential markers of fetal lung maturity) detectable by MR spectroscopy. Materials and Methods: 1H high-resolution magic angle spinning (HR-MAS) spectroscopy was performed at 11.7T on 23 third-trimester amniotic fluid samples. Samples were analyzed quantitatively using 3-(trimethylsilyl)propionic- 2,2,3,3-d4 acid (TSP) as a reference. Four observers independently quantified eight metabolite regions (TSP, lactate doublet and quartet, alanine, citrate, creatinine, choline, and glucose) twice from anonymized, randomized spectra using a semiautomated software program. Results: Excellent inter- and intraobserver reproducibility was found for all metabolites. Intraclass correlation as a measure of interobserver agreement for the four readers ranged from 0.654 to 0.995. A high correlation of 0.973 was seen for choline in particular, a major component of surfactant. Pearson correlation as a measure of intraobserver reproducibility ranged from 0.478 to 0.999. Conclusion: Quantification of choline and other metabolite concentrations in amniotic fluid by high-resolution MR spectroscopy can be performed with high inter- and intraobserver reproducibility. Demonstration of reproducible metabolite concentration measurements is a critical first step in the search for biomarkers of fetal lung maturity.

Original languageEnglish (US)
Pages (from-to)1540-1545
Number of pages6
JournalJournal of Magnetic Resonance Imaging
Volume28
Issue number6
DOIs
StatePublished - Dec 2008
Externally publishedYes

Fingerprint

Amniotic Fluid
Choline
Spectrum Analysis
Lung
Magnetic Resonance Spectroscopy
Third Pregnancy Trimester
Surface-Active Agents
Alanine
Lactic Acid
Creatinine
Software
Biomarkers
Glucose
Acids

Keywords

  • Amniotic fluid
  • Fetal lung maturity
  • High resolution magic angle spinning (HR-MAS)
  • Reproducibility

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

1H HR-MAS spectroscopy for quantitative measurement of choline concentration in amniotic fluid as a marker of fetal lung maturity : Interand intraobserver reproducibility study. / Joe, Bonnie N.; Vahidi, Kiarash; Zektzer, Andrew; Chen, Mei Hsiu; Clifton, Matthew S.; Butler, Thomas; Keshari, Kayvan; Kurhanewicz, John; Coakley, Fergus; Swanson, Mark G.

In: Journal of Magnetic Resonance Imaging, Vol. 28, No. 6, 12.2008, p. 1540-1545.

Research output: Contribution to journalArticle

Joe, Bonnie N. ; Vahidi, Kiarash ; Zektzer, Andrew ; Chen, Mei Hsiu ; Clifton, Matthew S. ; Butler, Thomas ; Keshari, Kayvan ; Kurhanewicz, John ; Coakley, Fergus ; Swanson, Mark G. / 1H HR-MAS spectroscopy for quantitative measurement of choline concentration in amniotic fluid as a marker of fetal lung maturity : Interand intraobserver reproducibility study. In: Journal of Magnetic Resonance Imaging. 2008 ; Vol. 28, No. 6. pp. 1540-1545.
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AU - Vahidi, Kiarash

AU - Zektzer, Andrew

AU - Chen, Mei Hsiu

AU - Clifton, Matthew S.

AU - Butler, Thomas

AU - Keshari, Kayvan

AU - Kurhanewicz, John

AU - Coakley, Fergus

AU - Swanson, Mark G.

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N2 - Purpose: To determine the intra- and interobserver reproducibility of human amniotic fluid metabolite concentration measurements (including potential markers of fetal lung maturity) detectable by MR spectroscopy. Materials and Methods: 1H high-resolution magic angle spinning (HR-MAS) spectroscopy was performed at 11.7T on 23 third-trimester amniotic fluid samples. Samples were analyzed quantitatively using 3-(trimethylsilyl)propionic- 2,2,3,3-d4 acid (TSP) as a reference. Four observers independently quantified eight metabolite regions (TSP, lactate doublet and quartet, alanine, citrate, creatinine, choline, and glucose) twice from anonymized, randomized spectra using a semiautomated software program. Results: Excellent inter- and intraobserver reproducibility was found for all metabolites. Intraclass correlation as a measure of interobserver agreement for the four readers ranged from 0.654 to 0.995. A high correlation of 0.973 was seen for choline in particular, a major component of surfactant. Pearson correlation as a measure of intraobserver reproducibility ranged from 0.478 to 0.999. Conclusion: Quantification of choline and other metabolite concentrations in amniotic fluid by high-resolution MR spectroscopy can be performed with high inter- and intraobserver reproducibility. Demonstration of reproducible metabolite concentration measurements is a critical first step in the search for biomarkers of fetal lung maturity.

AB - Purpose: To determine the intra- and interobserver reproducibility of human amniotic fluid metabolite concentration measurements (including potential markers of fetal lung maturity) detectable by MR spectroscopy. Materials and Methods: 1H high-resolution magic angle spinning (HR-MAS) spectroscopy was performed at 11.7T on 23 third-trimester amniotic fluid samples. Samples were analyzed quantitatively using 3-(trimethylsilyl)propionic- 2,2,3,3-d4 acid (TSP) as a reference. Four observers independently quantified eight metabolite regions (TSP, lactate doublet and quartet, alanine, citrate, creatinine, choline, and glucose) twice from anonymized, randomized spectra using a semiautomated software program. Results: Excellent inter- and intraobserver reproducibility was found for all metabolites. Intraclass correlation as a measure of interobserver agreement for the four readers ranged from 0.654 to 0.995. A high correlation of 0.973 was seen for choline in particular, a major component of surfactant. Pearson correlation as a measure of intraobserver reproducibility ranged from 0.478 to 0.999. Conclusion: Quantification of choline and other metabolite concentrations in amniotic fluid by high-resolution MR spectroscopy can be performed with high inter- and intraobserver reproducibility. Demonstration of reproducible metabolite concentration measurements is a critical first step in the search for biomarkers of fetal lung maturity.

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