17-β Estradiol rapidly enhances extracellular signal-regulated kinase 2 phosphorylation in the rat brain

D. N. Bryant, M. A. Bosch, Oline Ronnekleiv, Daniel Dorsa

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Physiological doses of 17-β Estradiol (E2) rapidly induce mitogen-activated protein kinase (MAPK) phosphorylation in a variety of cell culture and tissue explant preparations. Rapid MAPK phosphorylation has been implicated as a critical step in estrogen's effects on neuronal activity, gene transcription and neuroprotection. The present series of in vivo experiments were designed to determine whether acute administration of estrogen rapidly increased extracellular signal-regulated protein kinase (ERK) 2 phosphorylation. Brains were harvested 20 min after a single i.p. injection of 15 μg/kg of 17-β or 17-α estradiol. Twelve brain structures were micro-dissected, homogenized and processed for Western blotting. E2-treated rats exhibited a statistically significant increase in ERK2 phosphorylation in the diagonal band of Broca, rostral nucleus accumbens, paraventricular nucleus, arcuate nucleus and anteromedial visual cortex. Administration of the same dose of 17-α estradiol did not enhance ERK phosphorylation in any of the brain regions examined. The in vivo data presented here extend previously published in vitro data indicating that E2 rapidly activates MAPK in primary neuronal cultures, explants and cell lines. These data also indicate that MAPK activation is a potential mediator of estrogens effects in some but not all estrogen receptor containing regions of the brain.

Original languageEnglish (US)
Pages (from-to)343-352
Number of pages10
JournalNeuroscience
Volume133
Issue number1
DOIs
StatePublished - 2005

Fingerprint

Mitogen-Activated Protein Kinase 1
Estradiol
Mitogen-Activated Protein Kinases
Phosphorylation
Brain
Estrogens
Diagonal Band of Broca
Arcuate Nucleus of Hypothalamus
Paraventricular Hypothalamic Nucleus
Extracellular Signal-Regulated MAP Kinases
Nucleus Accumbens
Visual Cortex
Estrogen Receptors
Protein Kinases
Cell Culture Techniques
Western Blotting
Cell Line
Injections
Genes

Keywords

  • Arcuate nucleus
  • Diagonal band of Broca
  • ERK2
  • in vivo
  • Nucleus accumbens
  • Rapid signaling

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

17-β Estradiol rapidly enhances extracellular signal-regulated kinase 2 phosphorylation in the rat brain. / Bryant, D. N.; Bosch, M. A.; Ronnekleiv, Oline; Dorsa, Daniel.

In: Neuroscience, Vol. 133, No. 1, 2005, p. 343-352.

Research output: Contribution to journalArticle

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