17β-estradiol treatment profoundly down-regulates gene expression in spinal cord tissue in mice protected from experimental autoimmune encephalomyelitis

Agata Matejuk, Jami Dwyer, Corwyn Hopke, Arthur A. Vandenbark, Halina Offner

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

It is now well documented that experimental autoimmune encephalomyeltitis (EAE) can be effectively prevented by estrogen therapy. Previously, we identified a limited set of genes that were altered in spleens of mice protected from EAE by 17β-estradiol (E2) treatment. As a continuation of these studies, we present here transcriptional changes in genes expressed in spinal cord tissue. The Affymetrix microarray system was used to screen more than 12,000 genes from E2-treated double transgenic (BV8S2 and AV4) female mice protected from EAE vs. control mice with severe EAE. We found that estrogen therapy had a profound inhibitory effect on the expressions of many immune-related genes in spinal cords. Estrogen significantly affected the transcription of 315 genes, 302 of which were down-regulated and only 13 that were up-regulated by ≥2.4 fold. A number of genes encoding the histocompatibility complex, cytokines/receptors, chemokines, adhesion molecules, and signal transduction proteins were strongly down-regulated (>20 fold) in estrogen-treated mice to levels similar to those of the spinal cord tissue from unmanipulated mice. The identification of genes with altered expression patterns in the spinal cords of estrogen-treated mice provides unique insight into the process that ultimately results in protection against EAE.

Original languageEnglish (US)
Pages (from-to)185-193
Number of pages9
JournalArchivum immunologiae et therapiae experimentalis
Volume51
Issue number3
StatePublished - Jul 21 2003

Keywords

  • 17β estradiol
  • EAE
  • Microarray
  • Spinal cord

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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