μ-Opioid agonist-stimulated [35S]GTPγ s binding in guinea pig hypothalamus: Effects of estrogen

Matthew J. Cunningham, Yuan Fang, Dana E. Selley, Martin J. Kelly

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

μ-Opioid receptors play a critical role in the regulation of the female reproductive cycle, and estrogen modulates the coupling of μ-opioid receptors to a potassium channel in the basal hypothalarnus (BH) of the female guinea pig. Therefore, we ascertained the distribution of μ-opioid receptors in the BH with autoradiography using the μ-opioid selective agonist [3H]DAMGO. In addition, we investigated the effects of estrogen on DAMGO- or the GABA(B) receptor agonist baclofen-stimulated [35S]GTPγS binding in the BH. Based on the high density of μ-opioid receptors, but the lack of effects of estrogen on [35S]GTPγS binding, we conclude that μ- opioid receptor interaction with its G-protein is not the target of estrogen's actions.

Original languageEnglish (US)
Pages (from-to)341-346
Number of pages6
JournalBrain research
Volume791
Issue number1-2
DOIs
StatePublished - Apr 27 1998

Keywords

  • Baclofen
  • DAMGO
  • Estrogen
  • G-protein
  • GTPγS
  • μ-Opioid receptor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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