β-secretase cleavage of the fly amyloid precursor protein is required for glial survival

Bonnie J. Bolkan, Tilman Triphan, Doris Kretzschmar

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

β-secretase (or BACE1) is the key enzyme in the production ofβ-amyloid (Aβ), which accumulates in the senile plaques characteristic for Alzheimer's disease. Consequently, the lack of BACE1 prevents β-processing of the amyloid precursor protein andAβ production, which made it a promising target for drug development. However, the loss of BACE1 is also detrimental, leading to myelination defects and altered neuronal activity, functions that have been associated with the cleavage of Neuregulin and a voltage-gated sodium channel subunit. Here we show that the Drosophila ortholog of BACE, dBACE, is required for glial survival. Cell-specific knockdown experiments reveal that this is a non-cell autonomous function, as a knockdown of dBACE in photoreceptor neurons leads to progressive degeneration of glia in their target zone, the lamina. Interestingly, this phenotype is suppressed by the loss of the fly amyloid precursor protein (APPL), whereas a secretion-deficient form of APPL enhances the degeneration. This shows that full-length APPL in neurons promotes the death of neighboring glial cells and thatβ-processing of APPL is needed to prevent glial death. These results therefore not only demonstrate a novel function for an APP protein in glia, but they also show this function specifically requires regulation byβ-cleavage.

Original languageEnglish (US)
Pages (from-to)16181-16192
Number of pages12
JournalJournal of Neuroscience
Volume32
Issue number46
DOIs
StatePublished - Nov 14 2012

ASJC Scopus subject areas

  • Neuroscience(all)

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