β-Lactamase activity in human pus

Richard Bryant, A. L. Rashad, J. A. Mazza, D. Hammond

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Pus was obtained from patients with polymicrobial intraabdominal abscesses or polymicrobial empyema. Physical and chemical characteristics of 12 specimens were examined, and bacterial isolates were enumerated. Pus supernatant of six specimens rapidly inactivated penicillin cephalothin, and cefazolin. Carbenicillin and ticarcillin were similarly degraded by supernatant of certain pus specimens. Cefoxitin, chloramphenicol, and clindamycin were not appreciably inactivated by pus supernatant. Degradation of penicillin and cephalosporin congeners in pus was due to the presence of β-lactamase, as shown by chemical interaction with nitrocefin, chromatography, and inhibition by the β-lactamase inhibitor clavulanic acid. Pus supernatant containing β-lactamase activity reduced the bactericidal activity of carbenicillin against Bacteroides fragilis in whole pus in an abscess model in vitro. Bactericidal activity of clindamycin or cefoxitin was not impaired in pus containing β-lactamase.

Original languageEnglish (US)
Pages (from-to)594-601
Number of pages8
JournalJournal of Infectious Diseases
Volume142
Issue number4
StatePublished - 1980

Fingerprint

Suppuration
Human Activities
Carbenicillin
Cefoxitin
Clindamycin
Penicillins
Abscess
Ticarcillin
Cephalothin
Cefazolin
Bacteroides fragilis
Clavulanic Acid
Empyema
Chloramphenicol
Cephalosporins
Chromatography

ASJC Scopus subject areas

  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Bryant, R., Rashad, A. L., Mazza, J. A., & Hammond, D. (1980). β-Lactamase activity in human pus. Journal of Infectious Diseases, 142(4), 594-601.

β-Lactamase activity in human pus. / Bryant, Richard; Rashad, A. L.; Mazza, J. A.; Hammond, D.

In: Journal of Infectious Diseases, Vol. 142, No. 4, 1980, p. 594-601.

Research output: Contribution to journalArticle

Bryant, R, Rashad, AL, Mazza, JA & Hammond, D 1980, 'β-Lactamase activity in human pus', Journal of Infectious Diseases, vol. 142, no. 4, pp. 594-601.
Bryant R, Rashad AL, Mazza JA, Hammond D. β-Lactamase activity in human pus. Journal of Infectious Diseases. 1980;142(4):594-601.
Bryant, Richard ; Rashad, A. L. ; Mazza, J. A. ; Hammond, D. / β-Lactamase activity in human pus. In: Journal of Infectious Diseases. 1980 ; Vol. 142, No. 4. pp. 594-601.
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