β-Adrenergic receptor kinase-like activity and β-arrestin are expressed in osteoblastic cells

Michael Bliziotes, James Murtagh, Kristine Wiren

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Biologic responses to peptide calciotropic hormones, such as parathyroid hormone (PTH) and calcitonin, exhibit desensitization. As with most hormones, however, the mechanisms of desensitization are not completely understood. For the β2-adrenergic receptor (β2AR) system, which is coupled to adenylyl cyclase via the stimulatory guanine nucleotide-binding regulatory (G(s)) protein, homologous desensitization is mediated in part by a receptor- specific kinase (βARK) and a soluble cofactor (β-arrestin). Recently, this system has been reported to be involved in rapid homologous desensitization of the PTH/parathyroid hormone receptor protein (PTHrP) receptor. We have identified the presence of this system in bone using reverse-transcriptase PCR. Nucleotide sequence of PCR fragments from ROS 17/2.8 cells revealed 100% identity with rat brain βARK1 and β-arrestin 1 sequences. Northern analyses with RNA from ROS 17/2.8, UMR 106-H5 cells, and primary cultures of nontransformed neonatal rat calvariae demonstrated two mRNA species of 4 and 2.6 kilobases (kb) for βARK and 7.5 kb for β-arrestin, comparable to those found in bovine brain. βARK-like activity was demonstrated in cytosolic extracts of the UMR 106-H5 cells by assessing phosphorylation of the retinal photoreceptor, rhodopsin, by the extracts. Phosphorylation was enhanced with light-activated rhodopsin and by bovine brain G(βγ) subunits; heparin inhibited phosphorylation. These findings are characteristic of βARK. Expression of β-arrestin in the UMR 106-H5 cells was confirmed by immunoblot. Thus, osteoblastic cells express proteins, βARK, and β- arrestin, which may regulate desensitization of calciotropic hormone receptors.

Original languageEnglish (US)
Pages (from-to)820-826
Number of pages7
JournalJournal of Bone and Mineral Research
Volume11
Issue number6
StatePublished - Jun 1996

Fingerprint

Arrestin
Adrenergic Receptors
Phosphotransferases
Rhodopsin
Phosphorylation
Parathyroid Hormone
Brain
Parathyroid Hormone Receptor Type 1
Hormones
Vertebrate Photoreceptor Cells
Proteins
Primary Cell Culture
Guanine Nucleotides
Peptide Hormones
Calcitonin
Reverse Transcriptase Polymerase Chain Reaction
Adenylyl Cyclases
Skull
Heparin
RNA

ASJC Scopus subject areas

  • Surgery

Cite this

β-Adrenergic receptor kinase-like activity and β-arrestin are expressed in osteoblastic cells. / Bliziotes, Michael; Murtagh, James; Wiren, Kristine.

In: Journal of Bone and Mineral Research, Vol. 11, No. 6, 06.1996, p. 820-826.

Research output: Contribution to journalArticle

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